Stability of yeast cytoskeleton under cell stress

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F00%3A00003450" target="_blank" >RIV/00216224:14110/00:00003450 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Stability of yeast cytoskeleton under cell stress

Popis výsledku v původním jazyce

The effects of heat, osmotic and radiation stresses on cells exposed to a lethal heat shock were studied on the model yeast Saccharomyces cerevisiae CCY 21-4-59. Cell thermoresistance or thermosensitivity was detected by their ability to form colonies (CFU) at 25 degrees C. After the transfer from 25 to 46 degrees C most cells lost their viability after a 10-min incubation and this temperature was then referred to as lethal heat shock. A 30-min preincubation of cells at 37 or 41 degrees C (referred to here as a mild heat stress) led to an increase of their resistance to the lethal heat shock. Western blotting technique (+ ECL) revealed that during mild heat stress the concentration of Hsp 104 in cells increased, while in controls only traces of this protein were detected. The Hsp 104 synthesis was apparently adaptive, because cycloheximide present during the mild heat stress effectively prevented the increase of cell thermoresistance. Accumulation of Hsp 104 was detected also by indire

Název v anglickém jazyce

Stability of yeast cytoskeleton under cell stress

Popis výsledku anglicky

The effects of heat, osmotic and radiation stresses on cells exposed to a lethal heat shock were studied on the model yeast Saccharomyces cerevisiae CCY 21-4-59. Cell thermoresistance or thermosensitivity was detected by their ability to form colonies (CFU) at 25 degrees C. After the transfer from 25 to 46 degrees C most cells lost their viability after a 10-min incubation and this temperature was then referred to as lethal heat shock. A 30-min preincubation of cells at 37 or 41 degrees C (referred to here as a mild heat stress) led to an increase of their resistance to the lethal heat shock. Western blotting technique (+ ECL) revealed that during mild heat stress the concentration of Hsp 104 in cells increased, while in controls only traces of this protein were detected. The Hsp 104 synthesis was apparently adaptive, because cycloheximide present during the mild heat stress effectively prevented the increase of cell thermoresistance. Accumulation of Hsp 104 was detected also by indire

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GA204%2F00%2F0394" target="_blank" >GA204/00/0394: Buněčná stěna kvasinek jako extracelulární matrix: cytoskelet a stěnové funkce</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Folia Microbiologica

ISSN

0015-5632

e-ISSN

—

Svazek periodika

45

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

1

Strana od-do

—

Kód UT WoS článku

—

EID výsledku v databázi Scopus

—