CYTOSKELETON IN THE CELL CYCLE OF CRYPTOCOCCUS NEOFORMANS AND THE EFFECTS OF CYTOSKELETAL INHIBITORS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F01%3A00004338" target="_blank" >RIV/00216224:14110/01:00004338 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

CYTOSKELETON IN THE CELL CYCLE OF CRYPTOCOCCUS NEOFORMANS AND THE EFFECTS OF CYTOSKELETAL INHIBITORS

Popis výsledku v původním jazyce

The objectives of the study were (i) to identify morphologically distinct stages in the cell division cycle of C. neoformans, (ii) to identify microtubular and actin components of the cytoskeleton and (iii) to investigate the effect of two cytoskeletal inhibitors, Cytochalasin D and methylbenzimidazol-2-yl carbamate (MBC), on cell proliferation and potential cell death. Both interphase and M-phase were found to be characterised each by three distinct morphological stages: mother cell, development of a sterigma-like protrusion and bud emergence, and nuclear migration into the bud and subsequent mitosis, postmitotic phase, cytokinesis and cell separation. F-actin (patches, cables and cytokinetic rings) was similar to the ascomycetous budding yeast; patches accumulated at the apex of the growing sterigma, in the bud and around the septum, but also were evenly distributed in mother cells. Cytoplasmic and spindle microtubules were similar to those in the ascomycetous fission yeast. Cytoplas

Název v anglickém jazyce

CYTOSKELETON IN THE CELL CYCLE OF CRYPTOCOCCUS NEOFORMANS AND THE EFFECTS OF CYTOSKELETAL INHIBITORS

Popis výsledku anglicky

The objectives of the study were (i) to identify morphologically distinct stages in the cell division cycle of C. neoformans, (ii) to identify microtubular and actin components of the cytoskeleton and (iii) to investigate the effect of two cytoskeletal inhibitors, Cytochalasin D and methylbenzimidazol-2-yl carbamate (MBC), on cell proliferation and potential cell death. Both interphase and M-phase were found to be characterised each by three distinct morphological stages: mother cell, development of a sterigma-like protrusion and bud emergence, and nuclear migration into the bud and subsequent mitosis, postmitotic phase, cytokinesis and cell separation. F-actin (patches, cables and cytokinetic rings) was similar to the ascomycetous budding yeast; patches accumulated at the apex of the growing sterigma, in the bud and around the septum, but also were evenly distributed in mother cells. Cytoplasmic and spindle microtubules were similar to those in the ascomycetous fission yeast. Cytoplas

Druh

D - Stať ve sborníku

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F00%2F0391" target="_blank" >GA310/00/0391: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

XXIX.Výrocná konferencia o kvasinkách, Kongresové centrum SAV Smolenice

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

18-18

Název nakladatele

Chemický ústav Slovenské akademie ved, Bratislava

Místo vydání

Bratislava

Místo konání akce

Bratislava

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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