Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a lucia software

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F01%3A00005622" target="_blank" >RIV/00216224:14110/01:00005622 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a lucia software

Popis výsledku v původním jazyce

The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM), produced by Schwann cells and fibroblasts under control of the axons, is important extrinsic factor of the nerve regeneration. A different content of the endoneurial ECM can be expected in the dorsal and ventral spinal roots in relation to their axon type differences, but no information is available to the present time. In the present paper we demonstrated a comparison of immunofluorescenceintensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of the rat dorsal and ventral spinal roots. For this purpose the cryostat sections through the dorsal and ventral roots were cut and incubatedsimultaneously. We tested optimal incubation conditions with the primary and secondary antibodies to distinguish differences in immunostaining intensity of the endoneurial ECM for investigated molecules. The best results of distinct stai

Název v anglickém jazyce

Computer-assisted quantitative immunohistochemistry of the extracellular matrix in the dorsal and ventral spinal roots with the use of a lucia software

Popis výsledku anglicky

The dorsal and ventral spinal roots contain different types of axons. The endoneurial extracellular matrix (ECM), produced by Schwann cells and fibroblasts under control of the axons, is important extrinsic factor of the nerve regeneration. A different content of the endoneurial ECM can be expected in the dorsal and ventral spinal roots in relation to their axon type differences, but no information is available to the present time. In the present paper we demonstrated a comparison of immunofluorescenceintensity for chondroitin sulfate proteoglycan, fibronectin, tenascin and thrombospondin in the endoneurial ECM of the rat dorsal and ventral spinal roots. For this purpose the cryostat sections through the dorsal and ventral roots were cut and incubatedsimultaneously. We tested optimal incubation conditions with the primary and secondary antibodies to distinguish differences in immunostaining intensity of the endoneurial ECM for investigated molecules. The best results of distinct stai

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/GA309%2F00%2F0407" target="_blank" >GA309/00/0407: Celulární a extracelulární prostředí pro regeneraci axonů při kořenových a distálních lezích periferních nervů</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

The Histochemical Journal

ISSN

0018-2214

e-ISSN

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Svazek periodika

33

Číslo periodika v rámci svazku

8

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

475

Strana od-do

475

Kód UT WoS článku

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EID výsledku v databázi Scopus

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