Studium podmíněně patogenní lipofilní kvasinky Malassezia pachydermatis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F02%3A00011445" target="_blank" >RIV/00216224:14110/02:00011445 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

The Study of Potentially Pathogenic Lipophilic Yeast Malassezia pachydermatis

Popis výsledku v původním jazyce

The human potentially pathogenic yeast M. pachydermatis has been studied to identify cytoskeleton as possible therapeutic target for antifungal agents. The study by light and transmission electron microscopy (TEM) confirmed the small size of the cells (2,0-3,0 x 4,0-5,0 mm). Intracellular structures were not numerous, but with respect to the cell size they were relatively large. TEM of ultrathin sections and freeze-etched replicas confirmed the presence of thick compact cell wall, multilayered in the neck of budding cell. This thick cell wall appeared to be a barrier for fluorescent antibodies. The higher concentration of detergent and lytical wall enzymes, the prolonged time of their and fluorescent antibodies applications [1, 2] were necessary prerequisites to detect any microtubular and microfilamental structures, when compared to fission yeast [1]. Preliminary results showed relatively poor cytoskeletal structures. Contribution of this report is to confirm the ultrastructure of the

Název v anglickém jazyce

The Study of Potentially Pathogenic Lipophilic Yeast Malassezia pachydermatis

Popis výsledku anglicky

The human potentially pathogenic yeast M. pachydermatis has been studied to identify cytoskeleton as possible therapeutic target for antifungal agents. The study by light and transmission electron microscopy (TEM) confirmed the small size of the cells (2,0-3,0 x 4,0-5,0 mm). Intracellular structures were not numerous, but with respect to the cell size they were relatively large. TEM of ultrathin sections and freeze-etched replicas confirmed the presence of thick compact cell wall, multilayered in the neck of budding cell. This thick cell wall appeared to be a barrier for fluorescent antibodies. The higher concentration of detergent and lytical wall enzymes, the prolonged time of their and fluorescent antibodies applications [1, 2] were necessary prerequisites to detect any microtubular and microfilamental structures, when compared to fission yeast [1]. Preliminary results showed relatively poor cytoskeletal structures. Contribution of this report is to confirm the ultrastructure of the

Druh

D - Stať ve sborníku

CEP obor

FP - Ostatní lékařské obory

OECD FORD obor

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Projekt

<a href="/cs/project/GA310%2F00%2F0391" target="_blank" >GA310/00/0391: Cytoskelet u lidských patogenních kvasinkových mikroorganismů.</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2002

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

30nd ANNUAL CONFERENCE ON YEASTS

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

58-58

Název nakladatele

Yeast Commission, Czechoslovak Society for Microbiology

Místo vydání

Smolenice

Místo konání akce

Smolenice

Datum konání akce

1. 1. 2002

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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