Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F03%3A00008625" target="_blank" >RIV/00216224:14110/03:00008625 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury

Popis výsledku v původním jazyce

We focused on histochemical detection of distribution of NOR (argyrophylic nucleolar proteins) reflecting nucleolar integrity, immunohistochemical detection of MADD (mitogen activated death domain), a protein accumulated in nucleoli upon stimulation by ischemia and active form of caspase-3, a universal proteolytic enzyme of apoptosis. The terminal deoxynucleotidyl- transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) proved the presence of in situ DNA fragmentation. We used the modelof transient focal cerebral ischemia in rats. The period of ischemia lasted 15, 30, 60 and 120 minutes followed by 48 hrs of reperfusion. We examined the frontal lobe of the ipsilateral hemisphere. We found disintegrated nucleoli in all investigated periods of ischemia in cortical as well as subcortical neurons whereas the neurons of intact animals showed compact nucleoli with a few satellites. Nuclear positivity for MADD was apparent after 15 min. in neocortex and peaked after 30 min. o

Název v anglickém jazyce

Nucleolar segregation coincides with nuclear accumulation of death domain in neurons undergoing apoptosis induced by ischemia reperfusion injury

Popis výsledku anglicky

We focused on histochemical detection of distribution of NOR (argyrophylic nucleolar proteins) reflecting nucleolar integrity, immunohistochemical detection of MADD (mitogen activated death domain), a protein accumulated in nucleoli upon stimulation by ischemia and active form of caspase-3, a universal proteolytic enzyme of apoptosis. The terminal deoxynucleotidyl- transferase (TdT)-mediated dUTP-biotin nick-end-labeling method (TUNEL) proved the presence of in situ DNA fragmentation. We used the modelof transient focal cerebral ischemia in rats. The period of ischemia lasted 15, 30, 60 and 120 minutes followed by 48 hrs of reperfusion. We examined the frontal lobe of the ipsilateral hemisphere. We found disintegrated nucleoli in all investigated periods of ischemia in cortical as well as subcortical neurons whereas the neurons of intact animals showed compact nucleoli with a few satellites. Nuclear positivity for MADD was apparent after 15 min. in neocortex and peaked after 30 min. o

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Physiological Research

ISSN

0862-8408

e-ISSN

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Svazek periodika

xxx

Číslo periodika v rámci svazku

xx

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

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EID výsledku v databázi Scopus

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