Intra- a extraneuronální změny v imunofluorescenční lokalizaci prozánětlivých a protizánětlivých molekul v experimentálních modelech neuropatické bolesti

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F05%3A00012984" target="_blank" >RIV/00216224:14110/05:00012984 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain.

Popis výsledku v původním jazyce

Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the studyof immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well asnaive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at thesurface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following scia

Název v anglickém jazyce

Intra- and extraneuronal changes of immunofluorescence staining for inflammation-related molecules in the experimental models of peripheral neuropathic pain.

Popis výsledku anglicky

Peripheral neuropathic pain has been investigated using rat peripheral nerve injury models including unilateral sciatic nerve transection or ligation, spinal nerve ligation or ventral root transection. The experimental models were utilized for the studyof immunofluorescence (IF) staining for IL-1b, IL-6, TNFa and TNFaR1 in the rat DRG. Quantitative and qualitative IF changes in the DRG affected by unilateral nerve injury were compared with the contralateral ones removed from operated animals as well asnaive rats. Ipsi- and contralateral DRG displayed increased IF for TNFa and TNFaR1 when compared with DRG of naive rats. The TNFa IF was increased in both DRG neurons and their satellite glial cells, while enhanced IF for TNFaR1 was observed only at thesurface of satellite glial cells after various types of nerve injury. In addition, ipsilateral DRG exhibited an elevation of TNFa immunoreactivity by invaded ED-1+ macrophages. The IL-1b IF was increased in the DRG neurons following scia

Druh

D - Stať ve sborníku

CEP obor

FH - Neurologie, neurochirurgie, neurovědy

OECD FORD obor

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Projekt

<a href="/cs/project/GA309%2F03%2F1199" target="_blank" >GA309/03/1199: Změny v satelitních gliových buňkách a jejich extracelulární matrix po axotomii - podmínky pro tvorbu sympatických kolaterál ve spinálních gangliích ?</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2005

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Folia Medica Cassoviensia

ISBN

80-7097-607-1

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

22-22

Název nakladatele

Institute of Neurobiology, Slovak Academy of Science

Místo vydání

Košice

Místo konání akce

Tatranská Lomnica-Stará Lesná

Datum konání akce

1. 1. 2005

Typ akce podle státní příslušnosti

EUR - Evropská akce

Kód UT WoS článku

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