Functional polymer hydrogels for embryonic stem cell support

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F06%3A00028366" target="_blank" >RIV/00216224:14110/06:00028366 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Functional polymer hydrogels for embryonic stem cell support

Popis výsledku v původním jazyce

One of the most exciting challenges in development of transplantation therapies is to repair the damaged part of the organ or tissue by transplantation of undifferentiated embryonic stem (ES) cells or their differentiated derivatives within three-dimensional polymer scaffold. To address this issue, new cationic hydrogel slabs were synthesized by bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) with ethylene dimethacrylate (EDMA) or 1-vinyl-2-pyrrolidone (VP) with N,N'-divinylethyleneurea (DVEU) or EDMA in the presence of saccharose or NaCl as a porogen. Biocompatibility was studied with the use of feeder-independent mouse ES cell line D3. When cultured for a limited period of time, EScells retained their undifferentiated state independently of properties of the hydrogel slabs. Notably, prolonged culture in superporous hydrogel slabs initiated ES cell differentiation.

Název v anglickém jazyce

Functional polymer hydrogels for embryonic stem cell support

Popis výsledku anglicky

One of the most exciting challenges in development of transplantation therapies is to repair the damaged part of the organ or tissue by transplantation of undifferentiated embryonic stem (ES) cells or their differentiated derivatives within three-dimensional polymer scaffold. To address this issue, new cationic hydrogel slabs were synthesized by bulk radical copolymerization of 2-hydroxyethyl methacrylate (HEMA) and 2-(dimethylamino)ethyl methacrylate (DMAEMA) with ethylene dimethacrylate (EDMA) or 1-vinyl-2-pyrrolidone (VP) with N,N'-divinylethyleneurea (DVEU) or EDMA in the presence of saccharose or NaCl as a porogen. Biocompatibility was studied with the use of feeder-independent mouse ES cell line D3. When cultured for a limited period of time, EScells retained their undifferentiated state independently of properties of the hydrogel slabs. Notably, prolonged culture in superporous hydrogel slabs initiated ES cell differentiation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CD - Makromolekulární chemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2006

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biomedical Materials Research

ISSN

1549-3296

e-ISSN

—

Svazek periodika

76

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

—

Kód UT WoS článku

000234834700010

EID výsledku v databázi Scopus

—