Functional analysis of the susceptibility haplotype in the Receptor for Advanced Glycation End-products gene and its role in the hyperglycemia-driven pathology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00024278" target="_blank" >RIV/00216224:14110/08:00024278 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Functional analysis of the susceptibility haplotype in the Receptor for Advanced Glycation End-products gene and its role in the hyperglycemia-driven pathology

Popis výsledku v původním jazyce

Background and Aims: Receptor for Advanced Glycation End-products (RAGE) belongs to the class of membrane receptors recognizing AGEs produced in accelerated rate during hyperglycemia and contributing to the development of diabetic complications. Our previous studies have shown that common haplotype in the RAGE gene (denoted RAGE2) conferred susceptibility to the development and accelerated onset of diabetic nephropathy (frequency 21.7% in nephropathy vs. 12.8% in control diabetic, P=0.015, OR=1.65 [95%CI 1.08-2.5]). The aim of the study was to elucidate the functional role of the RAGE2 susceptibility haplotype in the modulation of RAGE gene transcriptional activity. Materials and Methods: A 788bp PCR fragment of the RAGE promoter region (-738 to +49 relative to the transcription start containing the -429T/C and -374T/A polymorphic variants) was amplified using genomic DNA of homozygous subjects to create all possible haplotypes present in population (i.e.

Název v anglickém jazyce

Functional analysis of the susceptibility haplotype in the Receptor for Advanced Glycation End-products gene and its role in the hyperglycemia-driven pathology

Popis výsledku anglicky

Background and Aims: Receptor for Advanced Glycation End-products (RAGE) belongs to the class of membrane receptors recognizing AGEs produced in accelerated rate during hyperglycemia and contributing to the development of diabetic complications. Our previous studies have shown that common haplotype in the RAGE gene (denoted RAGE2) conferred susceptibility to the development and accelerated onset of diabetic nephropathy (frequency 21.7% in nephropathy vs. 12.8% in control diabetic, P=0.015, OR=1.65 [95%CI 1.08-2.5]). The aim of the study was to elucidate the functional role of the RAGE2 susceptibility haplotype in the modulation of RAGE gene transcriptional activity. Materials and Methods: A 788bp PCR fragment of the RAGE promoter region (-738 to +49 relative to the transcription start containing the -429T/C and -374T/A polymorphic variants) was amplified using genomic DNA of homozygous subjects to create all possible haplotypes present in population (i.e.

Druh

O - Ostatní výsledky

CEP obor

FB - Endokrinologie, diabetologie, metabolismus, výživa

OECD FORD obor

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Projekt

<a href="/cs/project/KJB501620601" target="_blank" >KJB501620601: Funkční analýza rizikového haplotypu RAGE genu a jeho role v patogenezi hyperglykemií indukovaných změn</a><br>

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů