Funkční interakce integrinu beta 3 a AP-1 v diferencujících osteoklastech a dendritických buňkách

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F08%3A00024909" target="_blank" >RIV/00216224:14110/08:00024909 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Functional cross-talk of integrin b3 and AP-1 in differentiating osteoclasts and dendritic cells.

Popis výsledku v původním jazyce

Osteoclasts (OCL) and dendritic cells (DC) are highly specialized cells that originate from a common hematopoetic progenitor. The osteoclasts are involved in bone resorption; dendritic cells represent a key compound of immune system as antigen presentingcells. Despite distinct functions, similar signaling molecules are involved in control of differentiation of OCL and DC. These regulators include integrin b3, the ECM receptor, and transcription factors c-Jun and c-Fos (AP-1). To investigate the signaling crosstalk between Itgb3 and AP-1 in OCL and DC, we employed murine model of RAW264.7 macrophages. These cells differentiate to OCL or DC upon treatment with RANKL (Receptor activator of NFkB ligand)MCSF or LPS (Lipopolysacharide). Expression of itgb3,c-jun and c-fos as well as differentiation markers were determined at mRNA level by qRT-PCR or western blotting. Expression of itgb3 was induced by both RANKL MCSF and LPS; however, expression of c-fos and c-jun was regulated differently

Název v anglickém jazyce

Functional cross-talk of integrin b3 and AP-1 in differentiating osteoclasts and dendritic cells.

Popis výsledku anglicky

Osteoclasts (OCL) and dendritic cells (DC) are highly specialized cells that originate from a common hematopoetic progenitor. The osteoclasts are involved in bone resorption; dendritic cells represent a key compound of immune system as antigen presentingcells. Despite distinct functions, similar signaling molecules are involved in control of differentiation of OCL and DC. These regulators include integrin b3, the ECM receptor, and transcription factors c-Jun and c-Fos (AP-1). To investigate the signaling crosstalk between Itgb3 and AP-1 in OCL and DC, we employed murine model of RAW264.7 macrophages. These cells differentiate to OCL or DC upon treatment with RANKL (Receptor activator of NFkB ligand)MCSF or LPS (Lipopolysacharide). Expression of itgb3,c-jun and c-fos as well as differentiation markers were determined at mRNA level by qRT-PCR or western blotting. Expression of itgb3 was induced by both RANKL MCSF and LPS; however, expression of c-fos and c-jun was regulated differently

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2008

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Book of Abstracts

ISBN

978-83-927731-0-8

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

International Life Sciences Students's Conference

Místo vydání

Warsaw

Místo konání akce

Warsaw, Poland

Datum konání akce

10. 9. 2008

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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