Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00029527" target="_blank" >RIV/00216224:14110/09:00029527 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method

Popis výsledku v původním jazyce

The influence of ischemia and reperfusion duration on left ventricular depolarization is studied from electrograms (ECG) and monophasic action potentials (MAPs). Three orthogonal ECG signals and MAPs are recorded simultaneously during ischemia in isolated rabbit hearts perfused according to Langendorff. MAPs are recorded by optical method via fluorescence measurement from the heart surface at appointed place. The principle of optical measurement of MAP is an application of voltage-sensitive dye (VSD), di-4-ANEPPS in our experimental set-up, into the heart tissue. Optical signal therefore depends on cardiac action potential. The ratiometric fluorescence method, based on emitted light detection by a segment narrow-band photodiode at two different wavelengths, is used. MAPs are recorded with high signal-to-noise ratio and at 2kHz sampling frequency.

Název v anglickém jazyce

Influence of Ischemia and Reperfusion Duration on Left Ventricular Depolarization in Isolated Rabbit Hearts Registered by Optical Method

Popis výsledku anglicky

The influence of ischemia and reperfusion duration on left ventricular depolarization is studied from electrograms (ECG) and monophasic action potentials (MAPs). Three orthogonal ECG signals and MAPs are recorded simultaneously during ischemia in isolated rabbit hearts perfused according to Langendorff. MAPs are recorded by optical method via fluorescence measurement from the heart surface at appointed place. The principle of optical measurement of MAP is an application of voltage-sensitive dye (VSD), di-4-ANEPPS in our experimental set-up, into the heart tissue. Optical signal therefore depends on cardiac action potential. The ratiometric fluorescence method, based on emitted light detection by a segment narrow-band photodiode at two different wavelengths, is used. MAPs are recorded with high signal-to-noise ratio and at 2kHz sampling frequency.

Druh

O - Ostatní výsledky

CEP obor

ED - Fyziologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA102%2F07%2F1473" target="_blank" >GA102/07/1473: Optické metody registrace elektrických potenciálů a koncentrace vápníku v srdci s laserovou stabilizací</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů