Bone marrow versus peripheral blood: comparison of the chimerism analysis

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00036469" target="_blank" >RIV/00216224:14110/09:00036469 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Bone marrow versus peripheral blood: comparison of the chimerism analysis

Popis výsledku v původním jazyce

Background Bone marrow (BM) examination is routinely performed for minimal residual disease monitoring in leukemia patients. By contrast, chimerism is usually tested from peripheral blood (PB), and, surprisingly, little is known about the clinical impactof the chimerism status in BM. Patients and Methods 226 pairs of PB and BM samples obtained from 102 adult patients were investigated. Frequencies of diagnoses within samples were as follows: AML 42%, CML 24%, ALL 11%, CLL 7%, MDS 7%, other hematological malignancies 9%. Assessment of chimerism was performed using either capillary electrophoresis with fluorescence detection, or quantitative real-time PCR with one order of magnitude higher sensitivity of mixed chimerism detection. Results Mixed chimerism in BM was detected even more than 9 years after transplantation, however, on the other hand, complete donor chimerism was also observed as early as day +35.

Název v anglickém jazyce

Bone marrow versus peripheral blood: comparison of the chimerism analysis

Popis výsledku anglicky

Background Bone marrow (BM) examination is routinely performed for minimal residual disease monitoring in leukemia patients. By contrast, chimerism is usually tested from peripheral blood (PB), and, surprisingly, little is known about the clinical impactof the chimerism status in BM. Patients and Methods 226 pairs of PB and BM samples obtained from 102 adult patients were investigated. Frequencies of diagnoses within samples were as follows: AML 42%, CML 24%, ALL 11%, CLL 7%, MDS 7%, other hematological malignancies 9%. Assessment of chimerism was performed using either capillary electrophoresis with fluorescence detection, or quantitative real-time PCR with one order of magnitude higher sensitivity of mixed chimerism detection. Results Mixed chimerism in BM was detected even more than 9 years after transplantation, however, on the other hand, complete donor chimerism was also observed as early as day +35.

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

—

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů