HPLC-ECD determination of hydroxyl radicals in various experimental models

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F09%3A00051245" target="_blank" >RIV/00216224:14110/09:00051245 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

HPLC-ECD determination of hydroxyl radicals in various experimental models

Popis výsledku v původním jazyce

The production of hydroxyl radicals was evaluated as products of salicylic acid hydroxylation using HPLC with coulochemical detector. Two models were used. In human studies we evaluated the production of hydroxyl radicals in patients with cardiovasculardisease and in healthy volunteers. The determination of hydroxyl radicals in vivo was based on the evaluation of hydroxylation products of salicylic acid after the ingestion of acetylsalicylic acid (Aspirin). In animal studies we measured the productionof hydroxyl radicals in isolated rat and guinea pig hearts during ischemia-reperfusion injury. Isolated hearts were perfused with Krebs-Henseleit buffer containing 1mM salicylic acid according to the Langendorff method. The both methods above illustratea wide field of efficiency of HPLC methods coupled with coulometric detection for detection of the parameters of oxidative stress.

Název v anglickém jazyce

HPLC-ECD determination of hydroxyl radicals in various experimental models

Popis výsledku anglicky

The production of hydroxyl radicals was evaluated as products of salicylic acid hydroxylation using HPLC with coulochemical detector. Two models were used. In human studies we evaluated the production of hydroxyl radicals in patients with cardiovasculardisease and in healthy volunteers. The determination of hydroxyl radicals in vivo was based on the evaluation of hydroxylation products of salicylic acid after the ingestion of acetylsalicylic acid (Aspirin). In animal studies we measured the productionof hydroxyl radicals in isolated rat and guinea pig hearts during ischemia-reperfusion injury. Isolated hearts were perfused with Krebs-Henseleit buffer containing 1mM salicylic acid according to the Langendorff method. The both methods above illustratea wide field of efficiency of HPLC methods coupled with coulometric detection for detection of the parameters of oxidative stress.

Druh

O - Ostatní výsledky

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2009

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů