LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease

Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00040778" target="_blank" >RIV/00216224:14110/10:00040778 - isvavai.cz</a>
Výsledek na webu
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DOI - Digital Object Identifier
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Jazyk výsledku
angličtina
Název v původním jazyce
LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease
Popis výsledku v původním jazyce
The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.
Název v anglickém jazyce
LC-MS/MS Monitoring Human Plasma Proteins Potentionally related to Graft-versus-Host Disease
Popis výsledku anglicky
The aim of this study was to develop a reliable and sensitive method for determination of potential biomarkers in human plasma for early GVHD detection. Blood plasma was immunodepleted by MARS Hu-14 to remove fourteen most abundant proteins. The immunodepleted plasma samples were fractionated by LC capillary system on a reverse phase (mRP C18, Agilent) in water/acetonitrile gradient and subsequently digested with trypsin. The resulting peptides were analyzed using LC-MS/MS in autoMS/MS mode.

Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění
2010
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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