Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00040874" target="_blank" >RIV/00216224:14110/10:00040874 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/10:#0000547

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model

Popis výsledku v původním jazyce

The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 wa

Název v anglickém jazyce

Gene Expression of the Endothelin-1 in Vasospastic Flap Pedicle - an Experimental Study on a Porcine Model

Popis výsledku anglicky

The aim of this study was to evaluate the amount of Endothelin 1 (ET 1) gene expression in the vasospastic vessel of the flap pedicle to prove or disprove the role of ET 1 gene expression in pathogenesis of mechanically induced vasospasm The vasospasm was induced by the tension on the pedicle of the pedicled caudal superficial epigastric flap on 8 pigs Laser Doppler was used for peripheral blood flow measurement Specimens from the vasospastic vessel (B) and from the flap border with no vasospasm (A) were taken 2 h after the stimulus initiation Detection of ET 1 mRNA by Quantitative Real Time RI PCR was performed beta actin was selected as an acceptable reference gene. Relative gene expressions and time indicators of vasospasm were compared in both groups. In conclusion the expression of the ET 1 gene in the mechanically induced vasospastic vessel of the pedicled flap was not significantly increased In this study the causality of the vasospasm pathogenesis and gene expression of ET 1 wa

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

FJ - Chirurgie včetně transplantologie

OECD FORD obor

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Projekt

<a href="/cs/project/NR8368" target="_blank" >NR8368: Spazmy cév mikrochirurgicky přenášených laloků a možnosti jejich ovlivnění</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

ACTA VETERINARIA BRNO

ISSN

0001-7213

e-ISSN

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Svazek periodika

79

Číslo periodika v rámci svazku

3

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

5

Strana od-do

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Kód UT WoS článku

000285269100016

EID výsledku v databázi Scopus

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