Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F10%3A00048590" target="_blank" >RIV/00216224:14110/10:00048590 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/62156489:43210/10:00173121

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Popis výsledku v původním jazyce

A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.

Název v anglickém jazyce

Validated method for bioactive lignans in Schisandra chinensis in vitro cultures using a solid phase extraction and a monolithic column application

Popis výsledku anglicky

A simple and rapid method for determination of six lignans found in plant cell cultures of Schisandra chinensis was developed and validated. The lignans were extracted from plant samples with methanol and the extracts were effectively cleaned by solid-phase extraction using Strata C18-E (Phenomenex) cartridges. Chromatographic separation was carried out on a Chromolith Performance RP-18e monolithic column (100 x 4.6 mm, Merck) using an isocratic mobile phase of acetonitrile and water in a 50:50 (v/v) ratio. The eluent was monitored at 220 nm. The base line separation of schizandrin, gomisin A, deoxyschizandrin, gamma-schizandrin, gomisin N and wuweizisu C was achieved in a relatively short time period (20 minutes), which was made possible by the relatively high flow rate of the mobile phase (2 ml/min). The lower limit of quantitation was 0.1 mg/l for schizandrin and gomisin A, 0.3 mg/l for deoxyschizandrin, gamma-schizandrin, and gomisin N and 1 mg/l for wuweizisu C.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GA522%2F07%2F0995" target="_blank" >GA522/07/0995: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2010

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

BIOMEDICAL CHROMATOGRAPHY

ISSN

0269-3879

e-ISSN

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Svazek periodika

24

Číslo periodika v rámci svazku

9

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

7

Strana od-do

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Kód UT WoS článku

000281424600007

EID výsledku v databázi Scopus

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