Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F11%3A00056991" target="_blank" >RIV/00216224:14110/11:00056991 - isvavai.cz</a>

Výsledek na webu

<a href="http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf" target="_blank" >http://onlinelibrary.wiley.com/doi/10.1002/elps.201100430/pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201100430" target="_blank" >10.1002/elps.201100430</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients

Popis výsledku v původním jazyce

Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-20-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified.

Název v anglickém jazyce

Microfluidic tool based on the antibodymodified paramagnetic particles for detection of 8-hydroxy-20-deoxyguanosine in urine of prostate cancer patients

Popis výsledku anglicky

Guanosine derivatives are important for diagnosis of oxidative DNA damage including 8-hydroxy-20-deoxyguanosine (8-OHdG) as one of the most abundant products of DNA oxidation. This compound is commonly determined in urine, which makes 8-OHdG a good non-invasive marker of oxidation stress. In this study, we optimized and tested the isolation of 8-OHdG from biological matrix by using paramagnetic particles with an antibody-modified surface. 8-OHdG was determined using 1-naphthol generated by alkaline phosphatase conjugated with the secondary antibody. 1-Naphthol was determined by stopped flow injection analysis (SFIA) with electrochemical detector using a glassy carbon working electrode and by stationary electrochemical detection using linear sweep voltammetry. A special modular electrochemical SFIA system which needs only 10 mL of sample including working buffer for one analysis was completely designed and successfully verified.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

<a href="/cs/project/NS10200" target="_blank" >NS10200: Nádorové markery, jejich stanovení a korelace s karcinomem prostaty</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2011

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Electrophoresis

ISSN

0173-0835

e-ISSN

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Svazek periodika

32

Číslo periodika v rámci svazku

22

Stát vydavatele periodika

DE - Spolková republika Německo

Počet stran výsledku

14

Strana od-do

3207-3220

Kód UT WoS článku

000298098700018

EID výsledku v databázi Scopus

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