Effect of dibenzocyclooctadiene lignans on multidrug resistant promyelotic leukaemia cells
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F12%3A00057524" target="_blank" >RIV/00216224:14110/12:00057524 - isvavai.cz</a>
Výsledek na webu
<a href="http://biomed.papers.upol.cz/incpdfs/pri-990000-0200_10_26.pdf" target="_blank" >http://biomed.papers.upol.cz/incpdfs/pri-990000-0200_10_26.pdf</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Effect of dibenzocyclooctadiene lignans on multidrug resistant promyelotic leukaemia cells
Popis výsledku v původním jazyce
In this study we used multidrug resistant promyelotic leukaemia cells overexpressing MDR1 (P-glycoprotein), the most common member of ABC transporters family (HL60/ MDR). The ability to overcome multidrug resistance was examined in the panel of dibenzo[a,c]cyclooctadiene lignans, schizandrin, gomisin A, gomisin N, angeloylgomisin H, tigloylgomisin P, deoxyschizandrin-dicarbaldehyde, wuweizisu C, and S(-)- and R(+)-enantiomers of deoxyschizandrin, y-schizandrin and gomisin J. The lignans were isolated from Schisandra chinensis seeds or prepared semisynthetically. We observed that resistant HL60/MDR was nearly hundred times more resistant to doxorubicin than the parental line HL60; although both cell lines were similarly sensitive to lignans treatment, indicating that the lignans are not exported from the resistant cells. Using doxorubicin accumulation assay we demonstrated that all lignans significantly enhanced the accumulation of doxorubicin in drug resistant cells.
Název v anglickém jazyce
Effect of dibenzocyclooctadiene lignans on multidrug resistant promyelotic leukaemia cells
Popis výsledku anglicky
In this study we used multidrug resistant promyelotic leukaemia cells overexpressing MDR1 (P-glycoprotein), the most common member of ABC transporters family (HL60/ MDR). The ability to overcome multidrug resistance was examined in the panel of dibenzo[a,c]cyclooctadiene lignans, schizandrin, gomisin A, gomisin N, angeloylgomisin H, tigloylgomisin P, deoxyschizandrin-dicarbaldehyde, wuweizisu C, and S(-)- and R(+)-enantiomers of deoxyschizandrin, y-schizandrin and gomisin J. The lignans were isolated from Schisandra chinensis seeds or prepared semisynthetically. We observed that resistant HL60/MDR was nearly hundred times more resistant to doxorubicin than the parental line HL60; although both cell lines were similarly sensitive to lignans treatment, indicating that the lignans are not exported from the resistant cells. Using doxorubicin accumulation assay we demonstrated that all lignans significantly enhanced the accumulation of doxorubicin in drug resistant cells.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
FH - Neurologie, neurochirurgie, neurovědy
OECD FORD obor
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Návaznosti výsledku
Projekt
<a href="/cs/project/GA522%2F07%2F0995" target="_blank" >GA522/07/0995: Regulace biosyntézy sekundarních metabolitů v buněčné kultuře Schisandra chinensis</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2012
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů