Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00065980" target="_blank" >RIV/00216224:14110/13:00065980 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/13:00060810

Výsledek na webu

<a href="http://dx.doi.org/10.5966/sctm.2012-0081" target="_blank" >http://dx.doi.org/10.5966/sctm.2012-0081</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5966/sctm.2012-0081" target="_blank" >10.5966/sctm.2012-0081</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Popis výsledku v původním jazyce

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high andgradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as asingle-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system ishighly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.

Název v anglickém jazyce

Adaptation to robust monolayer expansion produces human pluripotent stem cells with improved viability

Popis výsledku anglicky

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high andgradually more viable than their feeder-dependent counterparts without changes attributed to culture adaptation. As a consequence, monolayer hPSCs possess advantages over their counterparts in embryoid body development, teratoma formation, freezing as asingle-cell suspension, and colony-forming efficiency. Importantly, this monolayer culture system is reversible, preserving the competence of hPSCs to gradually reacquire features of colony growth, if necessary. Therefore, the monolayer culture system ishighly suitable for long-term, large-scale propagation of hPSCs, which is necessary in drug development and pluripotent stem cell-based therapies.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

STEM CELLS Translational Medicine

ISSN

2157-6564

e-ISSN

—

Svazek periodika

2

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

9

Strana od-do

246-254

Kód UT WoS článku

000317551800002

EID výsledku v databázi Scopus

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