Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00068801" target="_blank" >RIV/00216224:14110/13:00068801 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216208:11130/13:10209746 RIV/00216305:26620/13:PU104424 RIV/00064203:_____/13:10209746 RIV/62156489:43210/13:43907731

Výsledek na webu

<a href="http://dx.doi.org/10.3892/or.2013.2389" target="_blank" >http://dx.doi.org/10.3892/or.2013.2389</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2013.2389" target="_blank" >10.3892/or.2013.2389</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

Popis výsledku v původním jazyce

Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition ofsarcosine (0; 10; 250; 500; 1,000 and 1,500 mu M) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells. We also determined metallothionein (MT) levels by chip capillary electrophoresis and Brdicka reaction in the cells treated with sarcosine. Sarcosine levels in the cells increased in aconcentration-dependent manner levels increased from only 270 nM with the lowest applied concentration of sarcosine (10 mu M) to 106 mu M with the highest applied concentration of sarcosine (1,500 mu M).

Název v anglickém jazyce

Effect of sarcosine on antioxidant parameters and metallothionein content in the PC-3 prostate cancer cell line

Popis výsledku anglicky

Sarcosine is currently one of the most discussed markers of prostate cancer. It is involved in amino acid metabolism and methylation processes that occur during the progression of prostate cancer. In this study, we monitored the effect of the addition ofsarcosine (0; 10; 250; 500; 1,000 and 1,500 mu M) in a time-dependent manner (0-72 h) on the PC-3 prostate cancer cell line. For the assessment of cell viability, the commonly used MTT test was employed. Furthermore, ion-exchange liquid chromatography was used for the determination of sarcosine content in the PC-3 cells. We also determined metallothionein (MT) levels by chip capillary electrophoresis and Brdicka reaction in the cells treated with sarcosine. Sarcosine levels in the cells increased in aconcentration-dependent manner levels increased from only 270 nM with the lowest applied concentration of sarcosine (10 mu M) to 106 mu M with the highest applied concentration of sarcosine (1,500 mu M).

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Oncology Reports

ISSN

1021-335X

e-ISSN

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Svazek periodika

29

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GR - Řecká republika

Počet stran výsledku

8

Strana od-do

2459-2466

Kód UT WoS článku

000319232800052

EID výsledku v databázi Scopus

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