Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F13%3A00073962" target="_blank" >RIV/00216224:14110/13:00073962 - isvavai.cz</a>

Výsledek na webu

—

DOI - Digital Object Identifier

—

Jazyk výsledku

angličtina

Název v původním jazyce

Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM

Popis výsledku v původním jazyce

Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders(van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy.

Název v anglickém jazyce

Improved IgH-based MRD Detection By Using Droplet Digital PCR: a Comparison With Real Time Quantitative PCR in MCL and MM

Popis výsledku anglicky

Minimal residual disease (MRD) detection is a well defined prognostic tool for response evaluation in hematological malignancies. Real time quantitative PCR (RQ-PCR) has become the settled and standardized method for MRD assessment in lymphoid disorders(van der Velden et al., Leukemia 2007). However, RQ-PCR based MRD detection relies on a relative quantification approach, since it requires a reference standard curve and an appropriate housekeeping gene for the normalization. Droplet Digital PCR (DD-PCR), applying the principle of limiting dilution of DNA and single molecule detection, allows reliable absolute tumor burden quantification at molecular level. In this study we performed a comparison of immunoglobulin heavy chain gene based MRD detection (IgH-MRD) performed by RQ-PCR and DD-PCR, to assess whether DD-PCR could overcome some RQ-PCR limitation and achieve superior sensitivity, robustness and accuracy.

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

—

Projekt

<a href="/cs/project/GAP304%2F10%2F1395" target="_blank" >GAP304/10/1395: Analýza klonálních progenitorů plazmatických buněk u monoklonálních gamapatií</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2013

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů