Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00074774" target="_blank" >RIV/00216224:14110/14:00074774 - isvavai.cz</a>
Nalezeny alternativní kódy
RIV/00216305:26620/14:PU108618 RIV/62156489:43210/14:43908675
Výsledek na webu
<a href="http://dx.doi.org/10.1002/elps.201300190" target="_blank" >http://dx.doi.org/10.1002/elps.201300190</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201300190" target="_blank" >10.1002/elps.201300190</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics
Popis výsledku v původním jazyce
Determination of serum mRNA gained a lot of attention in recent years, particularly from the perspective of disease markers. Streptavidin-modified paramagnetic particles (SMPs) seem an interesting technique, mainly due to possible automated isolation andhigh efficiency. The aim of this study was to optimize serum isolation protocol to reduce the consumption of chemicals and sample volume. The following factors were optimized: amounts of (i) paramagnetic particles, (ii) oligo(dT)20 probe, (iii) serum, and (iv) the binding sequence (SMPs, oligo(dT)20, serum vs. oligo(dT)20, serum and SMPs). RNA content was measured, and the expression of metallothionein-2A as possible prostate cancer marker was analyzed to demonstrate measurable RNA content with abilityfor RT-PCR detection.This optimized protocol was also modified to fit needs of automated one-step singletube analysis with identical efficiency compared to conventional setup.
Název v anglickém jazyce
Utilization of paramagnetic microparticles for automated isolation of free circulating mRNA as a new tool in prostate cancer diagnostics
Popis výsledku anglicky
Determination of serum mRNA gained a lot of attention in recent years, particularly from the perspective of disease markers. Streptavidin-modified paramagnetic particles (SMPs) seem an interesting technique, mainly due to possible automated isolation andhigh efficiency. The aim of this study was to optimize serum isolation protocol to reduce the consumption of chemicals and sample volume. The following factors were optimized: amounts of (i) paramagnetic particles, (ii) oligo(dT)20 probe, (iii) serum, and (iv) the binding sequence (SMPs, oligo(dT)20, serum vs. oligo(dT)20, serum and SMPs). RNA content was measured, and the expression of metallothionein-2A as possible prostate cancer marker was analyzed to demonstrate measurable RNA content with abilityfor RT-PCR detection.This optimized protocol was also modified to fit needs of automated one-step singletube analysis with identical efficiency compared to conventional setup.
Klasifikace
Druh
J<sub>x</sub> - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)
CEP obor
CE - Biochemie
OECD FORD obor
—
Návaznosti výsledku
Projekt
Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Ostatní
Rok uplatnění
2014
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Electrophoresis
ISSN
0173-0835
e-ISSN
—
Svazek periodika
35
Číslo periodika v rámci svazku
2-3
Stát vydavatele periodika
CZ - Česká republika
Počet stran výsledku
10
Strana od-do
306-315
Kód UT WoS článku
000331899400010
EID výsledku v databázi Scopus
—