The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00079025" target="_blank" >RIV/00216224:14110/14:00079025 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/68081707:_____/14:00441260 RIV/00159816:_____/14:00061063 RIV/61989592:15110/14:33163276 RIV/68081707:_____/14:00506380

Výsledek na webu

<a href="http://dx.doi.org/10.1186/1476-4598-13-113" target="_blank" >http://dx.doi.org/10.1186/1476-4598-13-113</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1186/1476-4598-13-113" target="_blank" >10.1186/1476-4598-13-113</a>

Jazyk výsledku

angličtina

Název v původním jazyce

The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Popis výsledku v původním jazyce

Background: Tumor heterogeneity and the plasticity of cancer cells present challenges for effective clinical diagnosis and therapy. Such challenges are epitomized by neuroendocrine transdifferentiation (NED) and the emergence of neuroendocrine-like cancer cells in prostate tumors. This phenomenon frequently arises from androgen-depleted prostate adenocarcinoma and is associated with the development of castration-resistant prostate cancer and poor prognosis.Results: In this study, we showed that NED wasevoked in both androgen receptor (AR)-positive and AR-negative prostate epithelial cell lines by growing the cells to a high density. Androgen depletion and high-density cultivation were both associated with cell cycle arrest and deregulated expression of several cell cycle regulators, such as p27Kip1, members of the cyclin D protein family, and Cdk2. Dual inhibition of Cdk1 and Cdk2 using pharmacological inhibitor or RNAi led to modulation of the cell cycle and promotion of NED.

Název v anglickém jazyce

The role of high cell density in the promotion of neuroendocrine transdifferentiation of prostate cancer cells

Popis výsledku anglicky

Background: Tumor heterogeneity and the plasticity of cancer cells present challenges for effective clinical diagnosis and therapy. Such challenges are epitomized by neuroendocrine transdifferentiation (NED) and the emergence of neuroendocrine-like cancer cells in prostate tumors. This phenomenon frequently arises from androgen-depleted prostate adenocarcinoma and is associated with the development of castration-resistant prostate cancer and poor prognosis.Results: In this study, we showed that NED wasevoked in both androgen receptor (AR)-positive and AR-negative prostate epithelial cell lines by growing the cells to a high density. Androgen depletion and high-density cultivation were both associated with cell cycle arrest and deregulated expression of several cell cycle regulators, such as p27Kip1, members of the cyclin D protein family, and Cdk2. Dual inhibition of Cdk1 and Cdk2 using pharmacological inhibitor or RNAi led to modulation of the cell cycle and promotion of NED.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2014

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Molecular Cancer

ISSN

1476-4598

e-ISSN

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Svazek periodika

13

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

16

Strana od-do

1-16

Kód UT WoS článku

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EID výsledku v databázi Scopus

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