Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084662" target="_blank" >RIV/00216224:14110/15:00084662 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1099/mic.0.000091" target="_blank" >http://dx.doi.org/10.1099/mic.0.000091</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1099/mic.0.000091" target="_blank" >10.1099/mic.0.000091</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae

Popis výsledku v původním jazyce

Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Burker Chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance ofactin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing beta-1,3-glucan microfibrils. The LA-inhibited cells lysed.

Název v anglickém jazyce

Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae

Popis výsledku anglicky

Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Burker Chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance ofactin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only 'table-mountain-like' wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing beta-1,3-glucan microfibrils. The LA-inhibited cells lysed.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Microbiology-SGM

ISSN

1350-0872

e-ISSN

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Svazek periodika

161

Číslo periodika v rámci svazku

"Part 7"

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

1348-1355

Kód UT WoS článku

000361411900006

EID výsledku v databázi Scopus

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