Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00084751" target="_blank" >RIV/00216224:14110/15:00084751 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/61389005:_____/15:00443231 RIV/61389013:_____/15:00443231 RIV/00159816:_____/15:00063806

Výsledek na webu

<a href="http://dx.doi.org/10.1021/bm501809t" target="_blank" >http://dx.doi.org/10.1021/bm501809t</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1021/bm501809t" target="_blank" >10.1021/bm501809t</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

Popis výsledku v původním jazyce

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated.

Název v anglickém jazyce

Toward Structured Macroporous Hydrogel Composites: Electron Beam-Initiated Polymerization of Layered Cryogels

Popis výsledku anglicky

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A I-125 radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CE - Biochemie

OECD FORD obor

—

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2015

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Biomacromolecules

ISSN

1525-7797

e-ISSN

—

Svazek periodika

16

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

11

Strana od-do

1146-1156

Kód UT WoS článku

000353006000008

EID výsledku v databázi Scopus

—