Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00088873" target="_blank" >RIV/00216224:14110/16:00088873 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/16:00065441

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients

Popis výsledku v původním jazyce

Multiple Myeloma (MM) remains incurable B-cell malignancy despite novel therapies. Plasmacytoid dendritic cells (pDC) have been shown to mediate tumour plasma cell growth, survival and drug resistance in MM patients. The role of effector gammadelta T cell interactions with pDC in patients progressing from monoclonal gammopathy of undetermined significance (MGUS) to MM is unknown. Our aim was to determine frequencies and phenotypes of pDC together with Vdelta1 and Vdelta2 gammadelta T cells. We used bone marrow (BM) and paired peripheral blood (PB) samples from MGUS (n=8) and newly diagnosed myeloma (n=11) patients. Total of n=24 age-matched healthy donors samples have been analysed. PDCs were analysed by multicolour flow cytometry as CD123+, CD303+, and CD304+. Phenotype of active pDC was identified as HLA-DR+ CD45RA+. In MGUS and MM patients, the medians of Vdelta1 T cells were comparable to normal levels in contrast to Vdelta2 T cells being significantly decreased in both patient cohorts.

Název v anglickém jazyce

Analysis of gamma-delta T cells and plasmacytoid dendritic cells in MGUS and Multiple Myeloma patients

Popis výsledku anglicky

Multiple Myeloma (MM) remains incurable B-cell malignancy despite novel therapies. Plasmacytoid dendritic cells (pDC) have been shown to mediate tumour plasma cell growth, survival and drug resistance in MM patients. The role of effector gammadelta T cell interactions with pDC in patients progressing from monoclonal gammopathy of undetermined significance (MGUS) to MM is unknown. Our aim was to determine frequencies and phenotypes of pDC together with Vdelta1 and Vdelta2 gammadelta T cells. We used bone marrow (BM) and paired peripheral blood (PB) samples from MGUS (n=8) and newly diagnosed myeloma (n=11) patients. Total of n=24 age-matched healthy donors samples have been analysed. PDCs were analysed by multicolour flow cytometry as CD123+, CD303+, and CD304+. Phenotype of active pDC was identified as HLA-DR+ CD45RA+. In MGUS and MM patients, the medians of Vdelta1 T cells were comparable to normal levels in contrast to Vdelta2 T cells being significantly decreased in both patient cohorts.

Druh

O - Ostatní výsledky

CEP obor

FD - Onkologie a hematologie

OECD FORD obor

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Projekt

<a href="/cs/project/NV15-32935A" target="_blank" >NV15-32935A: Analýza gamma-delta T lymfocytů reaktivních na nádorové buňky u pacientů s B-buněčnou chronickou lymfocytární leukémií: nový přístup k buněčné terapii</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů