PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

Kód výsledku v IS VaVaI

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RIV/68081731:_____/16:00460214

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

Popis výsledku v původním jazyce

Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascularnreplacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.nA method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. Thenpresented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.

Název v anglickém jazyce

PRINCIPAL COMPONENT ANALYSIS OF RAMAN SPECTROSCOPY DATA FOR DETERMINATION OF BIOFILM FORMING BACTERIA AND YEASTS

Popis výsledku anglicky

Many microorganisms (e.g., bacteria, yeast, and algae) are known to form a multi-layered structure composed of cells and extracellular matrix on various types of surfaces. Such a formation is known as the biofilm. Special attention is now paid to bacterial biofilms that are formed on the surface of medical implants, surgical fixations, and artificial tissue/vascularnreplacements. Cells contained within such a biofilm are well protected against antibiotics and phagocytosis and, thus, effectively resist antimicrobial attack.nA method for in vitro identification of individual bacterial cells as well as yeast colonies is presented. Figure 1 shows an an example of the biofilm formed by Staphylococcus epidermidis bacteria and Candida parapsilosis yeasts known for forming biofilms. Thenpresented method is based on analysis of spectral “Raman fingerprints” obtained from the single cell or whole colony, see figure 2(top). Here, Raman spectra might be taken from the biofilm-forming cells without the influence of an extracellular matrix or directly form the bacterial/yeast colony.

Druh

D - Stať ve sborníku

CEP obor

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OECD FORD obor

10306 - Optics (including laser optics and quantum optics)

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2016

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Recent Trends in Charged Particle Optics and Surface Physics Instrumentation

ISBN

9788087441176

ISSN

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e-ISSN

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Počet stran výsledku

2

Strana od-do

66-67

Název nakladatele

ACAD SCIENCES, CZECH REPUBLIC, INST SCIENTIFIC INSTRUMENTS ASCR, V V I

Místo vydání

BRNO

Místo konání akce

Skalský dvůr

Datum konání akce

29. 5. 2016

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

000391254000030