Association of serum N-epsilon-Carboxy methyl lysine with severity of diabetic retinopathy
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F16%3A00124650" target="_blank" >RIV/00216224:14110/16:00124650 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.sciencedirect.com/science/article/pii/S1056872715004912?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1056872715004912?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jdiacomp.2015.12.009" target="_blank" >10.1016/j.jdiacomp.2015.12.009</a>
Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
Association of serum N-epsilon-Carboxy methyl lysine with severity of diabetic retinopathy
Popis výsledku v původním jazyce
Purpose: To correlate serum levels of N-epsilon-carboxy methyl lysine (N-epsilon-CML) with severity of retinopathy, in vivo macular edema and disruption of external limiting membrane (ELM) and photoreceptor ellipsoid zone in type 2 diabetes mellitus (DM). Methods: Consecutive cases of type 2 DM [diabetes mellitus with no retinopathy (No DR) (n = 20); non proliferative diabetic retinopathy (NPDR) with diabetic macular edema (n = 20); proliferative diabetic retinopathy with diabetic macular edema (PDR) (n = 20)] and healthy controls (n = 20) between the ages of 40 and 65 years were included (power of study = 93.8%). In vivo histology of retinal layers was assessed using spectral domain optical coherence tomography. Every study subject underwent macular thickness analysis using the macular cube 512 x 128 feature. Disruption of ELM and photoreceptor ellipsoid zone was graded: grade 0, no disruption of ELM and ellipsoid zone; grade 1, ELM disrupted and ellipsoid zone intact; grade 2, both ELM and ellipsoid zone disrupted. Data were statistically analyzed. Results: The mean levels of N-epsilon-CML were 31.34 +/- 21.23 ng/ml, 73.88 +/- 35.01 ng/ml, 91.21 +/- 66.65 ng/ml, and 132.08 +/- 84.07 ng/ml in control, No DR, NPDR and PDR respectively. N-epsilon-CML level was significantly different between the study groups (control, No DR, NPDR and PDR) (p < 0.001). Mean logMAR visual acuity decreased with increased levels of N-epsilon-CML (p < 0.001). The association of (NCML)-C-epsilon with the grades of disruption was found to be statistically significant (F value = 18.48, p < 0.001). Univariate analysis was done with N-epsilon-CML as a dependent variable. The values of N-epsilon-CML were normalized (log(10)) and were subjected to univariate analysis with fasting blood glucose level, glycosylated hemoglobin, central subfield macular thickness and cube average thickness among the diseased groups (NPDR and PDR) that act as confounders. It was found that none of the variables had significant effect on N-epsilon-CML (fasting blood glucose p = 0.12, HBA1c p = 0.65, central subfield macular thickness p = 0.13, cube average thickness p = 0.19). N-epsilon-CML tends to be a significant and important predictor of grade of ELM and ellipsoid zone disruption in diabetic retinopathy. Conclusions: Increased N-epsilon-CML levels are associated with increased severity of diabetic retinopathy, macular edema and structural changes in macula that is ELM and ellipsoid zone disruption, which serves as a prognosticator of visual outcome. (C) 2016 Elsevier Inc. All rights reserved.
Název v anglickém jazyce
Association of serum N-epsilon-Carboxy methyl lysine with severity of diabetic retinopathy
Popis výsledku anglicky
Purpose: To correlate serum levels of N-epsilon-carboxy methyl lysine (N-epsilon-CML) with severity of retinopathy, in vivo macular edema and disruption of external limiting membrane (ELM) and photoreceptor ellipsoid zone in type 2 diabetes mellitus (DM). Methods: Consecutive cases of type 2 DM [diabetes mellitus with no retinopathy (No DR) (n = 20); non proliferative diabetic retinopathy (NPDR) with diabetic macular edema (n = 20); proliferative diabetic retinopathy with diabetic macular edema (PDR) (n = 20)] and healthy controls (n = 20) between the ages of 40 and 65 years were included (power of study = 93.8%). In vivo histology of retinal layers was assessed using spectral domain optical coherence tomography. Every study subject underwent macular thickness analysis using the macular cube 512 x 128 feature. Disruption of ELM and photoreceptor ellipsoid zone was graded: grade 0, no disruption of ELM and ellipsoid zone; grade 1, ELM disrupted and ellipsoid zone intact; grade 2, both ELM and ellipsoid zone disrupted. Data were statistically analyzed. Results: The mean levels of N-epsilon-CML were 31.34 +/- 21.23 ng/ml, 73.88 +/- 35.01 ng/ml, 91.21 +/- 66.65 ng/ml, and 132.08 +/- 84.07 ng/ml in control, No DR, NPDR and PDR respectively. N-epsilon-CML level was significantly different between the study groups (control, No DR, NPDR and PDR) (p < 0.001). Mean logMAR visual acuity decreased with increased levels of N-epsilon-CML (p < 0.001). The association of (NCML)-C-epsilon with the grades of disruption was found to be statistically significant (F value = 18.48, p < 0.001). Univariate analysis was done with N-epsilon-CML as a dependent variable. The values of N-epsilon-CML were normalized (log(10)) and were subjected to univariate analysis with fasting blood glucose level, glycosylated hemoglobin, central subfield macular thickness and cube average thickness among the diseased groups (NPDR and PDR) that act as confounders. It was found that none of the variables had significant effect on N-epsilon-CML (fasting blood glucose p = 0.12, HBA1c p = 0.65, central subfield macular thickness p = 0.13, cube average thickness p = 0.19). N-epsilon-CML tends to be a significant and important predictor of grade of ELM and ellipsoid zone disruption in diabetic retinopathy. Conclusions: Increased N-epsilon-CML levels are associated with increased severity of diabetic retinopathy, macular edema and structural changes in macula that is ELM and ellipsoid zone disruption, which serves as a prognosticator of visual outcome. (C) 2016 Elsevier Inc. All rights reserved.
Klasifikace
Druh
J<sub>imp</sub> - Článek v periodiku v databázi Web of Science
CEP obor
—
OECD FORD obor
30202 - Endocrinology and metabolism (including diabetes, hormones)
Návaznosti výsledku
Projekt
—
Návaznosti
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2016
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Údaje specifické pro druh výsledku
Název periodika
Journal of Diabetes and its Complications
ISSN
1056-8727
e-ISSN
1873-460X
Svazek periodika
30
Číslo periodika v rámci svazku
3
Stát vydavatele periodika
US - Spojené státy americké
Počet stran výsledku
7
Strana od-do
511-517
Kód UT WoS článku
000372940300021
EID výsledku v databázi Scopus
2-s2.0-84953439131