Role of formyl peptide receptor2 in schwannoma cell response during acute inflammation

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00100719" target="_blank" >RIV/00216224:14110/17:00100719 - isvavai.cz</a>

Výsledek na webu

<a href="http://www.morphology2017.cz/programme" target="_blank" >http://www.morphology2017.cz/programme</a>

DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Role of formyl peptide receptor2 in schwannoma cell response during acute inflammation

Popis výsledku v původním jazyce

ROLE OF FORMYL PEPTIDE RECEPTOR-2 IN SCHWANNOMA CELL RESPONSE DURING ACUTE INFLAMMATION Korimová A., Kohoutková M., Dubový P. Department of Anatomy, Faculty of Medicine, Masaryk University, Czech Republic 442669@mail.muni.cz Mitochondria contain formyl peptides that are normally hidden in cells but can be released into the circulation upon pathological injury. The mitochondrial formyl peptides are recognized by formyl peptide receptors (FPRs) involved in the pathogenesis of sterile and infectious inflammatory processes and also act as mitochondrial damage-associated patterns (DAMPs). Generally, FPRs (FPR1, FPR2 and FPR3) are expressed on the surface of many different cells type and initiate production of inflammatory cytokines by NFkB signaling pathway. We hypothesized that formyl peptides could be responsible for injury-mediated Schwann cell activation after peripheral nerve injury. The aim of this study was to investigate the immune response elicited by N-formyl-methionyl-leucyl-phenylalanine (N-fMLP)-activated Schwannoma cell line RT4-D6P2T and detect changes in FPR2 and cytokine expression. Protein expression profiles of FPR2 and localization in RT4-D6P2T cells were evaluated using immunocytochemistry and Western blot analysis. At 6 h N-fMLP treatment, significant increased prominent 38 kDa band expression of FPR2 occurred only at concentration 100nM N-fMLP. Furthermore, increasing the N-fMLP concentration had no significant influence on FPR2 expression and the weak activation observed in higher N-fMLP dose (10 µM, 50 µM) is considered as nonspecific dependent. Immunofluorescent labelling with anti-FPR2 antibody revealed the nuclear localization of FPR2. After 6 h post-treatment, IL-6 was significantly increased, although without differences in the dynamics of STAT3 poshophorylation and NFkB. Our result suggested that the FPR2-mediated responses are not only initiated at the cell surface and FPR2 could contribute to inflammatory profiling of Schwann cells. Supported by grant 16-08508S of The Czech Science Foundation

Název v anglickém jazyce

Role of formyl peptide receptor2 in schwannoma cell response during acute inflammation

Popis výsledku anglicky

ROLE OF FORMYL PEPTIDE RECEPTOR-2 IN SCHWANNOMA CELL RESPONSE DURING ACUTE INFLAMMATION Korimová A., Kohoutková M., Dubový P. Department of Anatomy, Faculty of Medicine, Masaryk University, Czech Republic 442669@mail.muni.cz Mitochondria contain formyl peptides that are normally hidden in cells but can be released into the circulation upon pathological injury. The mitochondrial formyl peptides are recognized by formyl peptide receptors (FPRs) involved in the pathogenesis of sterile and infectious inflammatory processes and also act as mitochondrial damage-associated patterns (DAMPs). Generally, FPRs (FPR1, FPR2 and FPR3) are expressed on the surface of many different cells type and initiate production of inflammatory cytokines by NFkB signaling pathway. We hypothesized that formyl peptides could be responsible for injury-mediated Schwann cell activation after peripheral nerve injury. The aim of this study was to investigate the immune response elicited by N-formyl-methionyl-leucyl-phenylalanine (N-fMLP)-activated Schwannoma cell line RT4-D6P2T and detect changes in FPR2 and cytokine expression. Protein expression profiles of FPR2 and localization in RT4-D6P2T cells were evaluated using immunocytochemistry and Western blot analysis. At 6 h N-fMLP treatment, significant increased prominent 38 kDa band expression of FPR2 occurred only at concentration 100nM N-fMLP. Furthermore, increasing the N-fMLP concentration had no significant influence on FPR2 expression and the weak activation observed in higher N-fMLP dose (10 µM, 50 µM) is considered as nonspecific dependent. Immunofluorescent labelling with anti-FPR2 antibody revealed the nuclear localization of FPR2. After 6 h post-treatment, IL-6 was significantly increased, although without differences in the dynamics of STAT3 poshophorylation and NFkB. Our result suggested that the FPR2-mediated responses are not only initiated at the cell surface and FPR2 could contribute to inflammatory profiling of Schwann cells. Supported by grant 16-08508S of The Czech Science Foundation

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/GA16-08508S" target="_blank" >GA16-08508S: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2017

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů