Increased levels of chemokine CCL2 and activation of neuronal regeneration program in the dorsal root ganglia neurons of both lumbar and cervical spinal segments after unilateral sciatic nerve lesion

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00101340" target="_blank" >RIV/00216224:14110/18:00101340 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Increased levels of chemokine CCL2 and activation of neuronal regeneration program in the dorsal root ganglia neurons of both lumbar and cervical spinal segments after unilateral sciatic nerve lesion

Popis výsledku v původním jazyce

Chemokine CCL2 acts through CCR2 and causes extravasation of activated macrophages into the inflamed areas. Moreover, it modulates inflammatory response in the injured nervous system and plays role in the activation of neuronal regeneration program. Surgical procedures were performed under aseptic conditions in 12 rats. The first group (CNI, n=4) underwent a compression of right sciatic nerve (NI), the NI of second group was cut (NIT, n =4). Rats were left to survive for 7 days. Naive rats (n=4) were used as control group. Rats were sacrificed and the dorsal root ganglia (DRG) were removed bilaterally in segments L4 and C7. CCL2, GAP43 and ED1+ macrophages were detected by indirect immunohistochemical method under the same conditions. The intensity of CCL2, GAP43 immunofluorescence (IF) and a ratio of ED1+ immunostained areas and areas containing the neuronal bodies were analyzed. The CCL2 and GAP43 IF intensity was significantly increased bilaterally in the DRG neurons of L4 and C7 segments after both CNI and NIT. The increase of CCL2 in the DRG neurons was followed by infiltration of activated ED1+ macrophages. The increase of CCL2 detected in all DRG neurons of L4 and C7 segments after both CNI and NIT with subsequent extravasation of activated ED1+ macrophages and enhanced levels of GAP43 suggested direct or indirect activation of the neuronal regeneration program also in the neurons of remote cervical DRG after peripheral nerve injury. Our results support the idea of systemic reaction of DRG neurons alongside neuroaxis to unilateral nerve injury.

Název v anglickém jazyce

Increased levels of chemokine CCL2 and activation of neuronal regeneration program in the dorsal root ganglia neurons of both lumbar and cervical spinal segments after unilateral sciatic nerve lesion

Popis výsledku anglicky

Chemokine CCL2 acts through CCR2 and causes extravasation of activated macrophages into the inflamed areas. Moreover, it modulates inflammatory response in the injured nervous system and plays role in the activation of neuronal regeneration program. Surgical procedures were performed under aseptic conditions in 12 rats. The first group (CNI, n=4) underwent a compression of right sciatic nerve (NI), the NI of second group was cut (NIT, n =4). Rats were left to survive for 7 days. Naive rats (n=4) were used as control group. Rats were sacrificed and the dorsal root ganglia (DRG) were removed bilaterally in segments L4 and C7. CCL2, GAP43 and ED1+ macrophages were detected by indirect immunohistochemical method under the same conditions. The intensity of CCL2, GAP43 immunofluorescence (IF) and a ratio of ED1+ immunostained areas and areas containing the neuronal bodies were analyzed. The CCL2 and GAP43 IF intensity was significantly increased bilaterally in the DRG neurons of L4 and C7 segments after both CNI and NIT. The increase of CCL2 in the DRG neurons was followed by infiltration of activated ED1+ macrophages. The increase of CCL2 detected in all DRG neurons of L4 and C7 segments after both CNI and NIT with subsequent extravasation of activated ED1+ macrophages and enhanced levels of GAP43 suggested direct or indirect activation of the neuronal regeneration program also in the neurons of remote cervical DRG after peripheral nerve injury. Our results support the idea of systemic reaction of DRG neurons alongside neuroaxis to unilateral nerve injury.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30103 - Neurosciences (including psychophysiology)

Projekt

<a href="/cs/project/GA16-08508S" target="_blank" >GA16-08508S: Zvýšení endogenního regeneračního programu a jeho aktivace zprostředkovaná cytokiny/chemokiny v neuronech ganglií bez spojení s poškozeným nervem</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů