Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00102960" target="_blank" >RIV/00216224:14110/18:00102960 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

Popis výsledku v původním jazyce

Cortisol is a glucocorticoid hormone, which plays an important role in stressful situations when stressors activate the hypothalamic-pituitary-adrenocortical (HPA) system. Cortisol has been routinely analyzed in the laboratory from saliva, serum/plasma, and/or urine. However, the HPA axis represents a highly dynamic system influenced by several factors and expose circadian rhythmicity. When analyzed in hair, it can allow the unique possibility to assess the long-term systemic levels and obtain a retrospective index of long-term hormone secretion. Based on an average hair growth rate of 1 cm per month, it is possible to distribute the hair along the length of individual segments and determine the concentration of cortisol reflecting secretion in the monitored period. Online SPE LC-MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.

Název v anglickém jazyce

Online solid-phase extraction liquid chromatography-mass spectrometry of hair cortisol using a surrogate analyte

Popis výsledku anglicky

Cortisol is a glucocorticoid hormone, which plays an important role in stressful situations when stressors activate the hypothalamic-pituitary-adrenocortical (HPA) system. Cortisol has been routinely analyzed in the laboratory from saliva, serum/plasma, and/or urine. However, the HPA axis represents a highly dynamic system influenced by several factors and expose circadian rhythmicity. When analyzed in hair, it can allow the unique possibility to assess the long-term systemic levels and obtain a retrospective index of long-term hormone secretion. Based on an average hair growth rate of 1 cm per month, it is possible to distribute the hair along the length of individual segments and determine the concentration of cortisol reflecting secretion in the monitored period. Online SPE LC-MS with 13C3-cortisol as a surrogate analyte was developed, validated and applied for obtaining hair cortisol levels of 114 healthy volunteers. The first 3 cm from a posterior vertex position were analyzed as three individual segments resulting in 310 hair cortisol levels, which were used, after log transformation, to predict a range of values observed in future. The median value of hair cortisol was 4.76 pg/mg. The employed method allowed simple processing, high throughput and may be readily expanded to analyze additional steroid compounds in hair.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10609 - Biochemical research methods

Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů