Radiofrequency ablation and transarterial chemoembolisation are characterized by changing dynamics of circulating microRNAs

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F18%3A00103797" target="_blank" >RIV/00216224:14110/18:00103797 - isvavai.cz</a>

Výsledek na webu

<a href="http://dx.doi.org/10.1007/s00270-018-2021-1" target="_blank" >http://dx.doi.org/10.1007/s00270-018-2021-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00270-018-2021-1" target="_blank" >10.1007/s00270-018-2021-1</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Radiofrequency ablation and transarterial chemoembolisation are characterized by changing dynamics of circulating microRNAs

Popis výsledku v původním jazyce

Purpose To investigate whether the expression level of circulating microRNAs related to hypoxia (miR-21 and miR-210), liver injury (miR-122) and epithelial-mesenchymal transition (miR-200a) could reflect changes in patients who underwent thermal ablation and chemoembolization. Material and methods This prospective study consisted of 30 patients diagnosed with hepatocellular carcinoma and liver metastases of colorectal cancer treated with thermal ablation (n=17) and chemoembolization (n=13). Thermal ablation was performed using a radiofrequency or microwave generator (RITA, Microsulis, AngioDynamics,Inc). For TACE, drug eluting beads (DCBeads, Biocompatibles Ltd.) were used. The tumour burden and treatment response was evaluated by RECIST, mRECIST and volumetry analyses. Four blood samples (before intervention, immediately after intervention, 24 hours after intervention, and control sample 1 week after intervention) were taken to measure plasmatic concentration of miRNA using miRNA-specific TaqMan assays and qRT-PCR method. Results In thermal ablation cases we observed a significant increase in investigated miRNA concentrations immediately after intervention (miR-122, FC=15, miR-200a, FC=1.9, P&lt;0.05). In TACE, we observed a delayed increase in circulating miRNA concentrations 24 hours after intervention (miR-21, FC=10.4, miR-210, FC=9.0, miR-122, FC=27, miR-200a, FC=4.0, P&lt;0.05). In both methods, the initial increase was followed by a steady decline of miRNA levels. Identified dynamic changes in circulating miRNA levels were in accordance with the nature of the thermal ablation and TACE hypoxia-related biological effects.The significant bias of tumour burden or treatment response was not shown in this sample size. Conclusion Our preliminary data indicates potential usage of circulating miRNAs for monitoring of the systemic effects of thermal ablative and intraarterial therapies.

Název v anglickém jazyce

Radiofrequency ablation and transarterial chemoembolisation are characterized by changing dynamics of circulating microRNAs

Popis výsledku anglicky

Purpose To investigate whether the expression level of circulating microRNAs related to hypoxia (miR-21 and miR-210), liver injury (miR-122) and epithelial-mesenchymal transition (miR-200a) could reflect changes in patients who underwent thermal ablation and chemoembolization. Material and methods This prospective study consisted of 30 patients diagnosed with hepatocellular carcinoma and liver metastases of colorectal cancer treated with thermal ablation (n=17) and chemoembolization (n=13). Thermal ablation was performed using a radiofrequency or microwave generator (RITA, Microsulis, AngioDynamics,Inc). For TACE, drug eluting beads (DCBeads, Biocompatibles Ltd.) were used. The tumour burden and treatment response was evaluated by RECIST, mRECIST and volumetry analyses. Four blood samples (before intervention, immediately after intervention, 24 hours after intervention, and control sample 1 week after intervention) were taken to measure plasmatic concentration of miRNA using miRNA-specific TaqMan assays and qRT-PCR method. Results In thermal ablation cases we observed a significant increase in investigated miRNA concentrations immediately after intervention (miR-122, FC=15, miR-200a, FC=1.9, P&lt;0.05). In TACE, we observed a delayed increase in circulating miRNA concentrations 24 hours after intervention (miR-21, FC=10.4, miR-210, FC=9.0, miR-122, FC=27, miR-200a, FC=4.0, P&lt;0.05). In both methods, the initial increase was followed by a steady decline of miRNA levels. Identified dynamic changes in circulating miRNA levels were in accordance with the nature of the thermal ablation and TACE hypoxia-related biological effects.The significant bias of tumour burden or treatment response was not shown in this sample size. Conclusion Our preliminary data indicates potential usage of circulating miRNAs for monitoring of the systemic effects of thermal ablative and intraarterial therapies.

Druh

O - Ostatní výsledky

CEP obor

—

OECD FORD obor

30224 - Radiology, nuclear medicine and medical imaging

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2018

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů