Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F19%3A00108020" target="_blank" >RIV/00216224:14110/19:00108020 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00159816:_____/19:00071035

Výsledek na webu

<a href="http://dx.doi.org/10.1038/s42003-019-0428-0" target="_blank" >http://dx.doi.org/10.1038/s42003-019-0428-0</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1038/s42003-019-0428-0" target="_blank" >10.1038/s42003-019-0428-0</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2

Popis výsledku v původním jazyce

Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activity. In cells, the total levels of the non-sumoylatable Dna2 variant are elevated. However, non-sumoylatable Dna2 shows impaired nuclear localization and reduced recruitment to foci upon DNA damage. Non-sumoylatable Dna2 reduces the rate of DNA end resection, as well as impedes cell growth and cell cycle progression through S phase. Taken together, these findings show that in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability.

Název v anglickém jazyce

Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2

Popis výsledku anglicky

Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activity. In cells, the total levels of the non-sumoylatable Dna2 variant are elevated. However, non-sumoylatable Dna2 shows impaired nuclear localization and reduced recruitment to foci upon DNA damage. Non-sumoylatable Dna2 reduces the rate of DNA end resection, as well as impedes cell growth and cell cycle progression through S phase. Taken together, these findings show that in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2019

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

COMMUNICATIONS BIOLOGY

ISSN

2399-3642

e-ISSN

—

Svazek periodika

2

Číslo periodika v rámci svazku

MAY 8 2019

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

12

Strana od-do

1-12

Kód UT WoS článku

000467215700004

EID výsledku v databázi Scopus

2-s2.0-85071051748