Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00114097" target="_blank" >RIV/00216224:14110/20:00114097 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/00216305:26230/19:PU135643

Výsledek na webu

<a href="https://link.springer.com/article/10.1007%2Fs12602-019-09548-3" target="_blank" >https://link.springer.com/article/10.1007%2Fs12602-019-09548-3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12602-019-09548-3" target="_blank" >10.1007/s12602-019-09548-3</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life

Popis výsledku v původním jazyce

Colinfant New Born (CNB) is an orally administered probiotic preparation containing theEscherichia colistrain A0 34/86, which is specially marketed for use in newborns and infants. Although the impact of different probiotics on the composition of the human gut microbiota has been previously described, the effects ofE. coliprobiotic consumption during infancy on the development of intestinal microbiota are not known. The effect of oral administration of CNB on theEnterobacteriaceaepopulation was mapped using 16S rRNA gene sequencing in DNA samples isolated from the stools of one infant collected at 177 different time points during the first year of life.E. colistrains turnover was analyzed based on the detection of 26 genetic determinants, phylogroups, and pulsed-field gel electrophoresis (PFGE) analysis. Administration of CNB during the second and third month of life introduced theEscherichiagenus to the infant's intestinal tract, andEscherichiabecame dominant among theEnterobacteriaceaefamily (p &lt; 0.01). Genetic determinants, typical for probioticE. coliA0 34/86 strain, were detected on the first day after application of CNB and persisted all year. In addition, nine transientE. colistrains were identified; these strains harbored different genetic determinants and showed different PFGE profiles. Transient strains were detected from 2 to 24 days in the stool samples. The firstEscherichiacolonizer originated from the application of the CNB probiotic preparation. ProbioticE. coliA0 34/86 successfully colonized the intestinal tract of an infant and became resident during the first year of life.

Název v anglickém jazyce

Administration of the Probiotic Escherichia coli Strain A0 34/86 Resulted in a Stable Colonization of the Human Intestine During the First Year of Life

Popis výsledku anglicky

Colinfant New Born (CNB) is an orally administered probiotic preparation containing theEscherichia colistrain A0 34/86, which is specially marketed for use in newborns and infants. Although the impact of different probiotics on the composition of the human gut microbiota has been previously described, the effects ofE. coliprobiotic consumption during infancy on the development of intestinal microbiota are not known. The effect of oral administration of CNB on theEnterobacteriaceaepopulation was mapped using 16S rRNA gene sequencing in DNA samples isolated from the stools of one infant collected at 177 different time points during the first year of life.E. colistrains turnover was analyzed based on the detection of 26 genetic determinants, phylogroups, and pulsed-field gel electrophoresis (PFGE) analysis. Administration of CNB during the second and third month of life introduced theEscherichiagenus to the infant's intestinal tract, andEscherichiabecame dominant among theEnterobacteriaceaefamily (p &lt; 0.01). Genetic determinants, typical for probioticE. coliA0 34/86 strain, were detected on the first day after application of CNB and persisted all year. In addition, nine transientE. colistrains were identified; these strains harbored different genetic determinants and showed different PFGE profiles. Transient strains were detected from 2 to 24 days in the stool samples. The firstEscherichiacolonizer originated from the application of the CNB probiotic preparation. ProbioticE. coliA0 34/86 successfully colonized the intestinal tract of an infant and became resident during the first year of life.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10606 - Microbiology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Probiotics and Antimicrobial Proteins

ISSN

1867-1306

e-ISSN

1867-1314

Svazek periodika

12

Číslo periodika v rámci svazku

2

Stát vydavatele periodika

US - Spojené státy americké

Počet stran výsledku

8

Strana od-do

343-350

Kód UT WoS článku

000541342000003

EID výsledku v databázi Scopus

2-s2.0-85065563862