Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00115637" target="_blank" >RIV/00216224:14110/20:00115637 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/65269705:_____/20:00072693

Výsledek na webu

<a href="https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract" target="_blank" >https://pubs.rsc.org/en/content/articlelanding/2020/AY/C9AY02383F#!divAbstract</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/c9ay02383f" target="_blank" >10.1039/c9ay02383f</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Popis výsledku v původním jazyce

Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value &lt;0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.

Název v anglickém jazyce

Application of an electrochemical LAMP-based assay for screening of HPV16/HPV18 infection in cervical samples

Popis výsledku anglicky

Cervical cancer is caused by a persistent infection with high-risk types of human papillomaviruses (HPVs), especially HPV16 and HPV18. HPV testing therefore represents an important alternative to standard cytological screening. However, existing HPV tests are often laborious, expensive or time-consuming, and thus novel techniques are developed that would be inexpensive, rapid, easy to perform and could be implemented in low-resource settings. In this work, we show an early application of an electrochemical (EC) assay combined with loop-mediated amplification (LAMP) to determine HPV16 and HPV18 status directly in a clinical material. To demonstrate its potential usefulness, we screened 61 cervical samples for the presence of both genotypes, and compared the results with the gold standard - PCR amplification followed by gel electrophoresis and two commercial HPV tests - COBAS and INNO-LiPA. The best concordance was obtained with the PCR reaction; we obtained high significance (p-value &lt;0.0001) and positive and negative predictive values over 90%. These data indicate that the EC-LAMP assay may serve as an interesting tool for rapid screening of oncogenic HPVs.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10401 - Organic chemistry

Projekt

—

Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Analytical Methods

ISSN

1759-9660

e-ISSN

1759-9679

Svazek periodika

12

Číslo periodika v rámci svazku

6

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

8

Strana od-do

822-829

Kód UT WoS článku

000514662700008

EID výsledku v databázi Scopus

2-s2.0-85079603578