A new simple approach to estimation of membrane capacitance from current responses to voltage clamp steps

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F20%3A00118630" target="_blank" >RIV/00216224:14110/20:00118630 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.sciencedirect.com/science/article/pii/S0079610720300341?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0079610720300341?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.pbiomolbio.2020.04.005" target="_blank" >10.1016/j.pbiomolbio.2020.04.005</a>

Jazyk výsledku

angličtina

Název v původním jazyce

A new simple approach to estimation of membrane capacitance from current responses to voltage clamp steps

Popis výsledku v původním jazyce

A variety of techniques of cell capacitance measurement have been proposed and applied in cellular electrophysiology. They are mostly based on the evaluation of membrane current responses to small changes in the membrane voltage. One of the currently used approaches applies the least-squares fit of an exponential current decay in response to voltage clamped rectangular pulses. In this study, we propose an alternative simpler approach to evaluation of the exponential parts in the current responses to square wave stimulation and present preliminary results of membrane capacitance evaluation. It is based on the property of the exponential function that has not yet been used to measure membrane capacitance. The time constant and the asymptote of the exponential waveform are unambiguously determined by the values read at three points separated by a constant time interval. In order to minimize the effect of noise and deviations from the exponential waveform, the triplet of points is designed to slide along the time axis. The results of the proposed approach and those previously evaluated by the least squares method are comparable. The method described may be advantageous for continuously recording changes in membrane capacitance. (C) 2020 Elsevier Ltd. All rights reserved.

Název v anglickém jazyce

A new simple approach to estimation of membrane capacitance from current responses to voltage clamp steps

Popis výsledku anglicky

A variety of techniques of cell capacitance measurement have been proposed and applied in cellular electrophysiology. They are mostly based on the evaluation of membrane current responses to small changes in the membrane voltage. One of the currently used approaches applies the least-squares fit of an exponential current decay in response to voltage clamped rectangular pulses. In this study, we propose an alternative simpler approach to evaluation of the exponential parts in the current responses to square wave stimulation and present preliminary results of membrane capacitance evaluation. It is based on the property of the exponential function that has not yet been used to measure membrane capacitance. The time constant and the asymptote of the exponential waveform are unambiguously determined by the values read at three points separated by a constant time interval. In order to minimize the effect of noise and deviations from the exponential waveform, the triplet of points is designed to slide along the time axis. The results of the proposed approach and those previously evaluated by the least squares method are comparable. The method described may be advantageous for continuously recording changes in membrane capacitance. (C) 2020 Elsevier Ltd. All rights reserved.

Druh

J_imp - Článek v periodiku v databázi Web of Science

CEP obor

—

OECD FORD obor

10608 - Biochemistry and molecular biology

Projekt

<a href="/cs/project/NV16-30571A" target="_blank" >NV16-30571A: Klinický význam a elektrofyziologické zhodnocení mutace c.926C>T genu KCNQ1 (p.T309I) jako možné „founder mutation“ syndromu dlouhého intervalu QT</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2020

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY

ISSN

0079-6107

e-ISSN

—

Svazek periodika

157

Číslo periodika v rámci svazku

NOV 2020

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

6

Strana od-do

18-23

Kód UT WoS článku

000582745400004

EID výsledku v databázi Scopus

2-s2.0-85086374991