Intact Cell Mass Spectrometry for Embryonic Stem Cell Biotyping

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F21%3A00120903" target="_blank" >RIV/00216224:14110/21:00120903 - isvavai.cz</a>

Výsledek na webu

<a href="https://www.intechopen.com/online-first/intact-cell-mass-spectrometry-for-embryonic-stem-cell-biotyping" target="_blank" >https://www.intechopen.com/online-first/intact-cell-mass-spectrometry-for-embryonic-stem-cell-biotyping</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.5772/intechopen.95074" target="_blank" >10.5772/intechopen.95074</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Intact Cell Mass Spectrometry for Embryonic Stem Cell Biotyping

Popis výsledku v původním jazyce

Stem cells represent a unique cell type that is capable of self-renewal and differentiation into somatic cell types. Since the derivation of human embryonic stem cells and induced pluripotent stem cells, enormous potential has been recognized for disease modeling, drug development and regenerative medicine. Both embryonic stem cells and induced pluripotent stem cells possess the ability to differentiate into all three germ layers, hence they are naturally prone to respond to various differentiation stimuli. These inherent cellular fluctuations, which can result in risky phenotypic instability, must be addressed prior to introduction of these cells to human medicine, since they represent one of the major biosafety obstacles in the development of bio-industrial or clinical-grade stem cell cultures. Therefore, there is an ongoing need for novel robust, feasible and sensitive methods for determination and confirmation of the otherwise identical cells status, as well as for the detection of hidden divergences from their optimal state. A method of choice can be the intact cell mass spectrometry. Here we show how it can be applied in routine quality control of embryonic stem cell cultures.

Název v anglickém jazyce

Intact Cell Mass Spectrometry for Embryonic Stem Cell Biotyping

Popis výsledku anglicky

Stem cells represent a unique cell type that is capable of self-renewal and differentiation into somatic cell types. Since the derivation of human embryonic stem cells and induced pluripotent stem cells, enormous potential has been recognized for disease modeling, drug development and regenerative medicine. Both embryonic stem cells and induced pluripotent stem cells possess the ability to differentiate into all three germ layers, hence they are naturally prone to respond to various differentiation stimuli. These inherent cellular fluctuations, which can result in risky phenotypic instability, must be addressed prior to introduction of these cells to human medicine, since they represent one of the major biosafety obstacles in the development of bio-industrial or clinical-grade stem cell cultures. Therefore, there is an ongoing need for novel robust, feasible and sensitive methods for determination and confirmation of the otherwise identical cells status, as well as for the detection of hidden divergences from their optimal state. A method of choice can be the intact cell mass spectrometry. Here we show how it can be applied in routine quality control of embryonic stem cell cultures.

Druh

C - Kapitola v odborné knize

CEP obor

—

OECD FORD obor

10406 - Analytical chemistry

Projekt

—

Návaznosti

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2021

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název knihy nebo sborníku

Mass Spectrometry in Life Sciences and Clinical Laboratory

ISBN

9781838818623

Počet stran výsledku

19

Strana od-do

133-151

Počet stran knihy

152

Název nakladatele

IntechOpen

Místo vydání

London

Kód UT WoS kapitoly

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