Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F22%3A00125709" target="_blank" >RIV/00216224:14110/22:00125709 - isvavai.cz</a>

Nalezeny alternativní kódy

RIV/60460709:41210/22:N0000098 RIV/65269705:_____/22:00075973

Výsledek na webu

<a href="https://sciendo.com/it/article/10.2478/acb-2022-0001" target="_blank" >https://sciendo.com/it/article/10.2478/acb-2022-0001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/acb-2022-0001" target="_blank" >10.2478/acb-2022-0001</a>

Jazyk výsledku

angličtina

Název v původním jazyce

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Popis výsledku v původním jazyce

The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-ra-pid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P &lt; 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.

Název v anglickém jazyce

Viability of bovine in vitro matured oocytes following ultra-rapid vitrification

Popis výsledku anglicky

The aim of the study was to examine viability of cattle oocytes after cryopreservation. Oocytes after in vitro maturation (IVM) were vitrified in minimum volume on the nickel electron microscopy grids by ultra-ra-pid cooling technique. After warming and subsequent in vitro fertilization the presumptive zygotes were cultured to reach the stage of the blastocyst (Bl). Several devitrified oocytes were processed for electron microscopy assay. Although, embryo cleavage and Bl percentages in the vitrified group were slightly lower than in the control group (P &lt; 0.05), the Bl total cell number (TCN), apoptosis and dead cell percentages did not differ between both groups. However, significant difference was found between day 7 (D7) and day 8 (D8) Bl in the TCN in control (108.0 vs. 90.5) and vitrified group (103.75 vs 98.14). Electron microscopy of frozen oocytes revealed slight reversible injuries in mitochondria and the smooth endoplasmic reticulum (SER), nevertheless, the development of devitrified oocytes to the Bl stage was comparable to those in fresh oocytes. In conclusion, higher proportion of slower developing Bl (D8) compared to D7 Bl may be related to the mentioned minor damages of some organelles in vitrified oocytes.

Druh

J_ost - Ostatní články v recenzovaných periodicích

CEP obor

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OECD FORD obor

30214 - Obstetrics and gynaecology

Projekt

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Návaznosti

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Rok uplatnění

2022

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Medical Journal of Cell Biology

ISSN

2544-3577

e-ISSN

2544-3577

Svazek periodika

10

Číslo periodika v rámci svazku

1

Stát vydavatele periodika

PL - Polská republika

Počet stran výsledku

6

Strana od-do

1-6

Kód UT WoS článku

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EID výsledku v databázi Scopus

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