Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F23%3A00131628" target="_blank" >RIV/00216224:14110/23:00131628 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells

Popis výsledku v původním jazyce

Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, diseasemodelling, and drug development. Their derivation is usually performed using a feeder layer, which isundefined and potentially contagious, and because of these difficulties, there is a tendency to replacefeeders with xeno-free defined substrates in recent years. We used truncated vitronectin for thederivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiatedstate, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers. These results support the conclusion that truncated vitronectin is a defined xeno-freesubstrate that is suitable for the derivation of hESCs.

Název v anglickém jazyce

Vitronectin is a xeno-free and defined surface for the derivation of human embryonic stem cells

Popis výsledku anglicky

Human embryonic stem cells (hESCs) have unique abilities that enable their use in cell therapy, diseasemodelling, and drug development. Their derivation is usually performed using a feeder layer, which isundefined and potentially contagious, and because of these difficulties, there is a tendency to replacefeeders with xeno-free defined substrates in recent years. We used truncated vitronectin for thederivation of hESCs for the first time, derived three hESC lines, and confirmed their undifferentiatedstate, hESC morphology, and standard karyotypes together with their potential to differentiate into three germ layers. These results support the conclusion that truncated vitronectin is a defined xeno-freesubstrate that is suitable for the derivation of hESCs.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10605 - Developmental biology

Projekt

<a href="/cs/project/NU22-08-00629" target="_blank" >NU22-08-00629: Vývoj postupů pro léčbu makulární degenerace sítnice deriváty lidských pluripotentních kmenových buněk</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2023

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů