OP12 - IN VITRO “GLICO” MODEL FOR STUDYING BRAIN CANCER PATHOGENESIS

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F24%3A00137761" target="_blank" >RIV/00216224:14110/24:00137761 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

OP12 - IN VITRO “GLICO” MODEL FOR STUDYING BRAIN CANCER PATHOGENESIS

Popis výsledku v původním jazyce

Advancements in preclinical models are pivotal for enhancing our understanding of complex diseases like glioblastoma. Our research team has developed a sophisticated in vitro model utilizing the co-culture of glioblastoma tumoroids and cerebral organoids (termed “GLICO”; Fig.1A), aiming to replicate the tumor microenvironment and investigate glioblastoma cell behavior. Leveraging extensive expertise in stem cell biology and neurodifferentiation, we have differentiated cerebral organoids from healthy induced pluripotent stem cells. We then integrated mature brain organoids with fluorescently labeled glioblastoma cell line U-87 (Fig.1B). This model now facilitates a detailed study of glioblastoma growth dynamics, and migration patterns (Fig.1C), and also allows us to specifically analyze glioblastoma cells after sorting. The pivotal findings, documented in our recent publication [1], underscore the model’s efficacy in mirroring the complex biological processes of glioblastoma, highlighted by significant alterations in glioblastoma cell fate upon interaction with the brain organoids. Efforts are underway to optimize this system for the co-culture of organoids with patients-derived tumoroids to provide a better gateway to discovering potential therapeutic targets and intervention strategies.

Název v anglickém jazyce

OP12 - IN VITRO “GLICO” MODEL FOR STUDYING BRAIN CANCER PATHOGENESIS

Popis výsledku anglicky

Advancements in preclinical models are pivotal for enhancing our understanding of complex diseases like glioblastoma. Our research team has developed a sophisticated in vitro model utilizing the co-culture of glioblastoma tumoroids and cerebral organoids (termed “GLICO”; Fig.1A), aiming to replicate the tumor microenvironment and investigate glioblastoma cell behavior. Leveraging extensive expertise in stem cell biology and neurodifferentiation, we have differentiated cerebral organoids from healthy induced pluripotent stem cells. We then integrated mature brain organoids with fluorescently labeled glioblastoma cell line U-87 (Fig.1B). This model now facilitates a detailed study of glioblastoma growth dynamics, and migration patterns (Fig.1C), and also allows us to specifically analyze glioblastoma cells after sorting. The pivotal findings, documented in our recent publication [1], underscore the model’s efficacy in mirroring the complex biological processes of glioblastoma, highlighted by significant alterations in glioblastoma cell fate upon interaction with the brain organoids. Efforts are underway to optimize this system for the co-culture of organoids with patients-derived tumoroids to provide a better gateway to discovering potential therapeutic targets and intervention strategies.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

10601 - Cell biology

Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2024

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů