DEFECTIVE TCR SURFACE EXPRESSION ASSOCIATED WITH IMPAIRED TCR BETA-CHAIN ASSEMBLY IN A PATIENT WITH CUTANEOUS T-CELL LYMPHOMA

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F95%3A00035802" target="_blank" >RIV/00216224:14110/95:00035802 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

DEFECTIVE TCR SURFACE EXPRESSION ASSOCIATED WITH IMPAIRED TCR BETA-CHAIN ASSEMBLY IN A PATIENT WITH CUTANEOUS T-CELL LYMPHOMA

Popis výsledku v původním jazyce

We report on a patient with cutaneous T-cell lymphoma (CTCL) of long-standing duration. Phenotypic analysis of his peripheral blood mononuclear cells revealed an increased CD4(+) T-helper subset and a decreased CD8(+) cytotoxic T-cell population. Eighty-three to ninety-three percent of the patient's CD4(+) T cells in the peripheral blood and 70% of the CD4(+) T cells in the lesional skin lacked surface expression of the TCR/CD3 complex and showed a clonal rearrangement pattern of the TCR gamma-chain gene (V11-J1/J2). The lack in TCR surface expression correlated with defective assembly of the TCR beta-chain. Although mRNA for the TCR constant region beta 1 was found in the patient's purified CD4(+)TCR(-)CD3(-) T cells, no intracytoplasmic TCR beta protein was detectable. In contrast, the patient's purified CD4(+)TCR(-)CD3(-) T cells not only expressed mRNA specific for the TCR alpha-chain and for all CD3 chains, but intracytoplasmic TCR alpha and CD3 epsilon proteins could also be foun

Název v anglickém jazyce

DEFECTIVE TCR SURFACE EXPRESSION ASSOCIATED WITH IMPAIRED TCR BETA-CHAIN ASSEMBLY IN A PATIENT WITH CUTANEOUS T-CELL LYMPHOMA

Popis výsledku anglicky

We report on a patient with cutaneous T-cell lymphoma (CTCL) of long-standing duration. Phenotypic analysis of his peripheral blood mononuclear cells revealed an increased CD4(+) T-helper subset and a decreased CD8(+) cytotoxic T-cell population. Eighty-three to ninety-three percent of the patient's CD4(+) T cells in the peripheral blood and 70% of the CD4(+) T cells in the lesional skin lacked surface expression of the TCR/CD3 complex and showed a clonal rearrangement pattern of the TCR gamma-chain gene (V11-J1/J2). The lack in TCR surface expression correlated with defective assembly of the TCR beta-chain. Although mRNA for the TCR constant region beta 1 was found in the patient's purified CD4(+)TCR(-)CD3(-) T cells, no intracytoplasmic TCR beta protein was detectable. In contrast, the patient's purified CD4(+)TCR(-)CD3(-) T cells not only expressed mRNA specific for the TCR alpha-chain and for all CD3 chains, but intracytoplasmic TCR alpha and CD3 epsilon proteins could also be foun

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

EC - Imunologie

OECD FORD obor

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Projekt

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Návaznosti

S - Specificky vyzkum na vysokych skolach

Rok uplatnění

1995

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

JOURNAL OF INVESTIGATIVE DERMATOLOGY

ISSN

0022-202X

e-ISSN

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Svazek periodika

104

Číslo periodika v rámci svazku

4

Stát vydavatele periodika

GB - Spojené království Velké Británie a Severního Irska

Počet stran výsledku

4

Strana od-do

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Kód UT WoS článku

A1995QR53200021

EID výsledku v databázi Scopus

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