Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F99%3A00003238" target="_blank" >RIV/00216224:14110/99:00003238 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

Popis výsledku v původním jazyce

Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identifyactin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportun istic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus.

Název v anglickém jazyce

Actin cytoskeleton in two human fungal pathogens Aureobasidium pullulans and Cryptococcus neoformans.

Popis výsledku anglicky

Two strains of human fungal pathogen Cryptococus neoformans and three strains of oportunistic pathogen Aureobasidium pullulans were examined by rhodamine-phalloidin and DAPI staining after paraformaldehyde fixation and Triton X-100 treatment to identifyactin cytoskeleton structures and nuclei.Uninuclear C. neoformans cells revealed actin cables oriented to developing bud, actin patches accumulated at growing bud and actin ring in the neck region between mother and daughter cells. In contrast, opportun istic pathogen A.pullulans did not reveal actin cables or patches, while actin rings were seen in multinuclear cells.However, all cells revealed diffuse F-actin cytoplasmic staining, accumulated at tips of young hyphae that may represent simple actin cytoskeleton enabling variable morphogenetic changes. Occurence of multinuclear cells in absence of actin cables supports our hypothesis on the role of actin cables in positioning of cell nucleus.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F97%2F1150" target="_blank" >GA204/97/1150: Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

20th International Specialized Symposium on Yeasts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Czech and Slovak Yeast Commission of the Czechoslovak Society for Microbiology

Místo vydání

Bratislava

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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