Restoration of the cytoskeleton during yeast protoplast regeneration

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F99%3A00003239" target="_blank" >RIV/00216224:14110/99:00003239 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Restoration of the cytoskeleton during yeast protoplast regeneration

Popis výsledku v původním jazyce

Restoration of actin cytoskeleton was studied using cells converted into protoplasts. In fresh protoplasts both cytoplasmic and nuclear microtubules desintegrated. Similarly actin cables lost their integrity while patches became evenly distributed underthe protoplast surface. A collapse of the cytoskeleton was caused by hypertonic stress when the cells were transferred to osmotic stabilizers. Microtubules and actin structures were renewed differently: cytoplasmic microtubules followed by nuclear microtubules appeared after 30-60 min of incubation. In S. cerevisiae and S. japonicus protoplasts actin appeared only in the form of patches:actin cables were seen neither in spherically nor polarly growing protoplasts. The cables appeared at the stage when the cell wall had been completely regenerated and a bud became apperent.

Název v anglickém jazyce

Restoration of the cytoskeleton during yeast protoplast regeneration

Popis výsledku anglicky

Restoration of actin cytoskeleton was studied using cells converted into protoplasts. In fresh protoplasts both cytoplasmic and nuclear microtubules desintegrated. Similarly actin cables lost their integrity while patches became evenly distributed underthe protoplast surface. A collapse of the cytoskeleton was caused by hypertonic stress when the cells were transferred to osmotic stabilizers. Microtubules and actin structures were renewed differently: cytoplasmic microtubules followed by nuclear microtubules appeared after 30-60 min of incubation. In S. cerevisiae and S. japonicus protoplasts actin appeared only in the form of patches:actin cables were seen neither in spherically nor polarly growing protoplasts. The cables appeared at the stage when the cell wall had been completely regenerated and a bud became apperent.

Druh

D - Stať ve sborníku

CEP obor

EE - Mikrobiologie, virologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA204%2F97%2F1150" target="_blank" >GA204/97/1150: Buněčná stěna a morfogeneze kvasinkové buňky: role cytoskeletu</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

1999

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

20th International Symposium on Yeasts

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

Czech and Slovak Commission of the Czechoslovak Society for Microbiology

Místo vydání

Bratislava

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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