SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
Identifikátory výsledku
Kód výsledku v IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F24%3A00139148" target="_blank" >RIV/00216224:14160/24:00139148 - isvavai.cz</a>
Výsledek na webu
<a href="https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB" target="_blank" >https://www.faf.cuni.cz/getattachment/Special/SAL2024/Program/SAL_2024_Book_of_Abstracts_v2.pdf.aspx?lang=en-GB</a>
DOI - Digital Object Identifier
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Alternativní jazyky
Jazyk výsledku
angličtina
Název v původním jazyce
SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
Popis výsledku v původním jazyce
Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability.
Název v anglickém jazyce
SYNTHESIS AND APPLICATION OF NOVEL FLUORESCENT LABELING TAGS FOR OLIGOSACCHARIDE AND GLYCAN ANALYSIS
Popis výsledku anglicky
Sample preparation is crucial for oligosaccharide and glycan analysis by chromatographic or electrophoretic methods. Since native oligosaccharides and glycans lack chromophores or fluorophores, they require molecular modification for optical detection. Adding a charged group also enhances electrophoretic mobility and ionization efficiency for MS detection. Standard oligosaccharides and N-linked glycans were derivatized with newly synthesized fluorescent and MS-active labels. After purification, the labeled compounds were separated by CE or LC and detected using fluorescence or MS. Novel labels based on 2-phenylpyridine derivatives were created, allowing tuning of fluorescence and MS properties. Compared to commercially available labels, these new labels showed higher reaction yields, tunable fluorescence, and better ionization efficiency. This work showcases the development of novel fluorescent labels with fast reaction kinetics, high fluorescence yields, and improved MS detectability.
Klasifikace
Druh
O - Ostatní výsledky
CEP obor
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OECD FORD obor
10406 - Analytical chemistry
Návaznosti výsledku
Projekt
<a href="/cs/project/GA22-00236S" target="_blank" >GA22-00236S: Vývoj nových fluorescenčních značek pro elektroforetické analýzy glykoproteinů asociovaných s rakovinou prsu</a><br>
Návaznosti
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Ostatní
Rok uplatnění
2024
Kód důvěrnosti údajů
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů