Isolation of new sesquiterpene lactones of Schkuhria pinnata Lam., Asteraceae

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14160%2F25%3A00143728" target="_blank" >RIV/00216224:14160/25:00143728 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Isolation of new sesquiterpene lactones of Schkuhria pinnata Lam., Asteraceae

Popis výsledku v původním jazyce

S. pinnata contains a variety of biologically active components, mainly sesquiterpene lactones, some acylated phenylpropanoids, flavonoids, and phytosterols.2 We focused on the separation of the ethyl acetate extract. The extract was subjected to a silica gel column chromatography, yielding 88 fractions. Based on HPLC analysis, they were combined into 26 fractions. Fractions SCH_H_E_32-42, SCH_H_E_43-47 and SCH_H_E_70-71 were selected for further separation using semi-preparative HPLC. This procedure led to the isolation of eight compounds. After evaluating the results by spectroscopic methods and comparing them with the literature, the following substances were identified as pectolinarigenin, hispidulin, and four sesquiterpene lactones - schkuhrin I, schkuhrin II, hiyodorilactone B and 2-dehydroeucannabinolide semiacetal.2 Two additional compounds were identified as novel sesquiterpene lactones,of germacranolide types, heliangolides (compounds 1 and 2) (unpublished data). Isolated sesquiterpene lactones have been subjected to cytotoxicity assays, as well as tests for antiproliferative and pro-apoptotic activity.

Název v anglickém jazyce

Isolation of new sesquiterpene lactones of Schkuhria pinnata Lam., Asteraceae

Popis výsledku anglicky

S. pinnata contains a variety of biologically active components, mainly sesquiterpene lactones, some acylated phenylpropanoids, flavonoids, and phytosterols.2 We focused on the separation of the ethyl acetate extract. The extract was subjected to a silica gel column chromatography, yielding 88 fractions. Based on HPLC analysis, they were combined into 26 fractions. Fractions SCH_H_E_32-42, SCH_H_E_43-47 and SCH_H_E_70-71 were selected for further separation using semi-preparative HPLC. This procedure led to the isolation of eight compounds. After evaluating the results by spectroscopic methods and comparing them with the literature, the following substances were identified as pectolinarigenin, hispidulin, and four sesquiterpene lactones - schkuhrin I, schkuhrin II, hiyodorilactone B and 2-dehydroeucannabinolide semiacetal.2 Two additional compounds were identified as novel sesquiterpene lactones,of germacranolide types, heliangolides (compounds 1 and 2) (unpublished data). Isolated sesquiterpene lactones have been subjected to cytotoxicity assays, as well as tests for antiproliferative and pro-apoptotic activity.

Druh

O - Ostatní výsledky

CEP obor

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OECD FORD obor

30104 - Pharmacology and pharmacy

Projekt

<a href="/cs/project/GA23-04655S" target="_blank" >GA23-04655S: Role prenylace a glykosylace v protizánětlivé aktivitě a metabolismu přírodních fenolových látek</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Rok uplatnění

2025

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů