Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00002294" target="_blank" >RIV/00216224:14310/00:00002294 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

Popis výsledku v původním jazyce

On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in theirrestriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective,

Název v anglickém jazyce

Preparation of specific probes detecting prophages of serogroups A, B and F in lysogenic strains of Staphylococcus aureus

Popis výsledku anglicky

On the basis of quantitative HindIII-restriction analysis of genomic DNAs, the S. aureus bacteriophages of International Typing Set were divided into five clusters designated as A, F, Ba, Bb and Bc. The clusters A and F include all the phages of serogroups A and F and correspond with the species 3A and 77 proposed by Ackermann and DuBow (1987). On the other hand, the phages of serogroup B were divided into three clusters designated as Ba, Bb and Bc that differ significantly each from the other in theirrestriction pattern. The clusters Ba and Bb may represent two separate species, while the cluster Bc, formed at a low similarity level, may include more than one phage species. For each of the phage serogroups A, B and F, the common HindIII-restriction fragments were identified of which those of phage 3A (1700 bp), of 53 (4060 bp) and of 77 (8300 bp) were used for the preparation of probes specific to the phages of serogroups A, B and F. These probes have been shown to be very effective,

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

<a href="/cs/project/GA301%2F99%2FD075" target="_blank" >GA301/99/D075: Genotypová diagnostika a typizace klinicky významných koaguláza negativních stafylokoků</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

9th International Symposium on Staphylococci and Staphylococcal Infections

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

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Název nakladatele

JDS

Místo vydání

Kolding

Místo konání akce

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Datum konání akce

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Typ akce podle státní příslušnosti

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Kód UT WoS článku

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