Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00004626" target="_blank" >RIV/00216224:14310/00:00004626 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology

Popis výsledku v původním jazyce

Fumigation-extraction method (FEM) is one of the main methodical implements of current soil microbiology. FEM serves to quantify microbial biomass as carbon immobilized in microorganisms - Cbio. Principle of the method is dispatching of soil microorganism cells with fumigant (redestilled chloroform). Biomass is obtained from 0.5M K2SO4 extractable carbon difference from fumigated and non-fumigated samples. Study was focused on biochemical specification of soil organic matter extracted 0.5M K2SO4 beforeand after fumigation. Colorimetric analytical methods were found to be the most suitable alternative for this purpose. The organic C (Cox), phenol-, anthrone- and orcinol-reactive organic compounds (PRC, ARC and ORC), ninhydrin-reactive nitrogen (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC) and diphenylamine-reactive compounds (DRC) were evaluated using extracts from 48 different soils. All of the used methods were quick, simple and cheap. Parameters obtained from such measur

Název v anglickém jazyce

Methodical progress of microbial biomass determination with classical fumigation-extraction method - further possible improvement in soil microbial ecotoxicology

Popis výsledku anglicky

Fumigation-extraction method (FEM) is one of the main methodical implements of current soil microbiology. FEM serves to quantify microbial biomass as carbon immobilized in microorganisms - Cbio. Principle of the method is dispatching of soil microorganism cells with fumigant (redestilled chloroform). Biomass is obtained from 0.5M K2SO4 extractable carbon difference from fumigated and non-fumigated samples. Study was focused on biochemical specification of soil organic matter extracted 0.5M K2SO4 beforeand after fumigation. Colorimetric analytical methods were found to be the most suitable alternative for this purpose. The organic C (Cox), phenol-, anthrone- and orcinol-reactive organic compounds (PRC, ARC and ORC), ninhydrin-reactive nitrogen (NRN), Folin-Ciocalteu's reagent-reactive compounds (FRC) and diphenylamine-reactive compounds (DRC) were evaluated using extracts from 48 different soils. All of the used methods were quick, simple and cheap. Parameters obtained from such measur

Druh

D - Stať ve sborníku

CEP obor

EH - Ekologie – společenstva

OECD FORD obor

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Projekt

<a href="/cs/project/GA526%2F98%2F1147" target="_blank" >GA526/98/1147: Diversita a funkční analýza půdních mikrobiálních společenstev v terestrických ekosystémech kontaminovaných persistentními organickými polutanty (POP)</a><br>

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2000

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Symposium Program of Prague 2000: Fifth International Symposium and Exhibition on Environmental Contamination in Central and Eastern Europe; 12-14 September 2000, Prague, Czech Republic

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

312

Strana od-do

144

Název nakladatele

Institute for International Cooperative Environmental Research

Místo vydání

Praha

Místo konání akce

Praha

Datum konání akce

1. 1. 2000

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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