Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Kód výsledku v IS VaVaI

RIV/00216224:14310/01:00003419 - isvavai.cz

Nalezeny alternativní kódy

RIV/68081707:_____/01:17013004

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Popis výsledku v původním jazyce

The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.

Název v anglickém jazyce

Thermal and thermodynamic properties of duplex DNA containing site-specific interstrand cross-link of antitumor cisplatin or its clinically ineffective trans isomer

Popis výsledku anglicky

The effect of the site-specific interstrand cross-link formed by cisplatin or transplatin on the thermal stability and energetics of 20-base pairs DNA duplex is reported. The cross-linked or unplatinated 20-bp duplexes were investigated with the aid of differential scanning calorimetry, temperature-dependent UV absorption and circular dichroism. The cross-link of both platinum isomers increases the thermal stability of the modified duplexes by changing the molecularity of denaturation. The structural perturbation due to the interstrand cross-link of cisplatin increases entropy of the duplex. By contrast, the interstrand cross-link of transplatin is enthalpically almost neutral. These differences are consistent with distinct conformational distortions induced by the interstrand cross-links of the two isomers. It has been proposed that the results of the present work might be associated with distinctly different antitumor effects of these platinum compounds.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

BO - Biofyzika

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Svazek periodika

276

Číslo periodika v rámci svazku

276

Stát vydavatele periodika

CZ - Česká republika

Počet stran výsledku

7

Strana od-do

9655

Kód UT WoS článku

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EID výsledku v databázi Scopus

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