Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00005395" target="_blank" >RIV/00216224:14310/01:00005395 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

Popis výsledku v původním jazyce

Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the si

Název v anglickém jazyce

Genotype variability of TSST-1 positive Staphylococcus aureus strains in the Czech Republic

Popis výsledku anglicky

Species Staphylococcus aureus belongs to clinically important gram-positive cocci. Some S. aureus strains produce TSST-1 toxin, which causes human disease called Toxic Shock Syndrom (TSS). Two forms of TSS were differentiated: the first type occurs in connection with menses, the second one is formed of cases, when TSS is a complication of another staphylococcal disease, for exampte food poisoning. Data about abiGty of S. aureus strains to produce TSST-1 toxin are clearly different. Therefore, we analyzed genomic DNAs of 26 S. aureus strains isolated from patients in the Czech Republic in connection with TSS. Staphylococcal isolates were analyzed by PCR using specific oligonucleotide primers to confirm presence of specific sequences of tst gene. To determine genetic variability, we performed macrorestriction analysis using PFGE, which enables determination of 20 different PFGE types. Strains were divided into 8 PFGE groups (ET1-ET8) according to Smal restriction patteros based on the si

Druh

D - Stať ve sborníku

CEP obor

EB - Genetika a molekulární biologie

OECD FORD obor

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Projekt

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Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

V. Pracovní setkání biochemiků a molekulárních biologů

ISBN

80-210-2538-7

ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

25

Název nakladatele

Masarykova univerzita v Brně

Místo vydání

Brno

Místo konání akce

Brno

Datum konání akce

1. 1. 2001

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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