Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures interfered with hydrogen evolution

Kód výsledku v IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F01%3A00006052" target="_blank" >RIV/00216224:14310/01:00006052 - isvavai.cz</a>

Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures interfered with hydrogen evolution

Popis výsledku v původním jazyce

A new method for the simultaneous determination of adenine and cytosine is described. Multivariate calibration based on a suitable experimental design (ED) and soft modelling with artificial neural networks (ANNs) is used for quantitative analysis of overlapped linear scan voltammetric (LSV) and differential pulse polarographic (DPP) peaks of adenine and cytosine that occur in the region of hydrogen evolution. It is demonstrated that analysis of mixtures, even if some of the constituents undergo an irreversible reduction, can be quantified with reasonable accuracy. The average absolute error was estimated as 3.7 % in LSV, 4.6 % in DPP for adenine and 5.2 % in LSV, 5.9 % in DPP for cytosine. For the whole testing set the comparison of the added and found values of adenine and cytosine concentrations was characterised by an agreement factor (about 0.06). The method is quite general and can be used for analysis of other biologically important substances without their separation.

Název v anglickém jazyce

Use of artificial neural networks for the evaluation of electrochemical signals of adenine and cytosine in mixtures interfered with hydrogen evolution

Popis výsledku anglicky

A new method for the simultaneous determination of adenine and cytosine is described. Multivariate calibration based on a suitable experimental design (ED) and soft modelling with artificial neural networks (ANNs) is used for quantitative analysis of overlapped linear scan voltammetric (LSV) and differential pulse polarographic (DPP) peaks of adenine and cytosine that occur in the region of hydrogen evolution. It is demonstrated that analysis of mixtures, even if some of the constituents undergo an irreversible reduction, can be quantified with reasonable accuracy. The average absolute error was estimated as 3.7 % in LSV, 4.6 % in DPP for adenine and 5.2 % in LSV, 5.9 % in DPP for cytosine. For the whole testing set the comparison of the added and found values of adenine and cytosine concentrations was characterised by an agreement factor (about 0.06). The method is quite general and can be used for analysis of other biologically important substances without their separation.

Druh

J_x - Nezařazeno - Článek v odborném periodiku (Jimp, Jsc a Jost)

CEP obor

CB - Analytická chemie, separace

OECD FORD obor

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Projekt

<a href="/cs/project/GV204%2F97%2FK084" target="_blank" >GV204/97/K084: Elektrody modifikované nukleovými kyselinami a bílkovinami. Nové nástroje v biochemickém a biomedicínském výzkumu</a><br>

Návaznosti

Z - Vyzkumny zamer (s odkazem do CEZ)

Rok uplatnění

2001

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název periodika

J.Electroanal.Chem.

ISSN

0022-0728

e-ISSN

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Svazek periodika

2002

Číslo periodika v rámci svazku

1-2

Stát vydavatele periodika

NL - Nizozemsko

Počet stran výsledku

8

Strana od-do

117

Kód UT WoS článku

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EID výsledku v databázi Scopus

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