Determination of The Kinetic Parameters of Haloalkane Dehalogenase by EMMA In a Partially Filled Capillary

Kód výsledku v IS VaVaI

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Výsledek na webu

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DOI - Digital Object Identifier

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Jazyk výsledku

angličtina

Název v původním jazyce

Determination of The Kinetic Parameters of Haloalkane Dehalogenase by EMMA In a Partially Filled Capillary

Popis výsledku v původním jazyce

Halogenated aliphatic hydrocarbons constitute one of the largest groups of environmental pollutants as a result of their widespread use as solvents, pesticides, herbicides, insecticides and chemical intermediates. Because of their toxicity, bioconcentration and persistence, the ubiquitous distribution of halogenated compounds in the biosphere has caused public concern over the possible effects on the quality of life. Haloalkane dehalogenases [EC 3.8.1.5] are group of enzymes involved in the biodegradation of these compounds by catalysing cleavage of the carbon-halogen bond. The study of the biochemistry of dehalogenation processes may help to understand and evaluate the potential for their degradation in nature. Moreover, biotransformation of organic compounds with biocatalysts offers new routes for the synthesis of intermediates. This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of haloalkane dehalogenase. In

Název v anglickém jazyce

Determination of The Kinetic Parameters of Haloalkane Dehalogenase by EMMA In a Partially Filled Capillary

Popis výsledku anglicky

Halogenated aliphatic hydrocarbons constitute one of the largest groups of environmental pollutants as a result of their widespread use as solvents, pesticides, herbicides, insecticides and chemical intermediates. Because of their toxicity, bioconcentration and persistence, the ubiquitous distribution of halogenated compounds in the biosphere has caused public concern over the possible effects on the quality of life. Haloalkane dehalogenases [EC 3.8.1.5] are group of enzymes involved in the biodegradation of these compounds by catalysing cleavage of the carbon-halogen bond. The study of the biochemistry of dehalogenation processes may help to understand and evaluate the potential for their degradation in nature. Moreover, biotransformation of organic compounds with biocatalysts offers new routes for the synthesis of intermediates. This report describes the application of electrophoretically mediated microanalysis (EMMA) for the study of kinetic parameters of haloalkane dehalogenase. In

Druh

D - Stať ve sborníku

CEP obor

CE - Biochemie

OECD FORD obor

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Projekt

Výsledek vznikl pri realizaci vícero projektů. Více informací v záložce Projekty.

Návaznosti

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Rok uplatnění

2003

Kód důvěrnosti údajů

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Název statě ve sborníku

Proceeding of 27th Symposium on HPLC and Related Techniques

ISBN

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ISSN

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e-ISSN

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Počet stran výsledku

1

Strana od-do

133-133

Název nakladatele

MCI Paris France

Místo vydání

Nice, France

Místo konání akce

Nice, France

Datum konání akce

15. 6. 2003

Typ akce podle státní příslušnosti

WRD - Celosvětová akce

Kód UT WoS článku

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